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Antibodies

APOLIPOPROTEIN J Antibody

Product Sizes
500 ug
600-101-198-500UG
About this Product
SKU:
600-101-198
Additional Names:
CLU|goat anti-Apolipoprotein J antibody, Clusterin, Complement-associated protein SP-40, Complement cytolysis inhibitor, CLI, NA1/NA2, Apo-J, Testosterone-repressed prostate message 2, TRPM-2, Ku70-binding protein 1, Aging-associated gene 4 protein, Clusterin beta chain, Clusterin alpha chain, Complement cytolysis inhibitor a chain, Complement cytolysis inhibitor b chain, ApoJ alpha, ApoJ beta, CLU, CLI, KUB1, AAG4
Application:
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Apolipoprotein J Antibody functions as a secreted chaperone that prevents aggregation of nonnative proteins. It prevents stress-induced aggregation of blood plasma proteins and inhibits the formations of amyloid fibrils. Apolipoprotein J does not require ATP or refold proteins by itself. It maintains partially unfolded proteins in a state for subsequent refolding by other chaperones. It is shown to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. Binding to cell surface receptors it triggers internalization of chaperone-client complex and subsequent lysosomal or proteasomal degradation. It modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis while mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Anti-Apolipoprotein J Antibody is useful for researchers interested in the immune system, Ubiquitin pathways, and cardiovascular research.
Formulation:
0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
Host:
Goat
Immunogen:
apoLipoprotein Type J was isolated from human plasma by density gradient centrifugation followed by HPLC purification.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Blue Ice
Specificity:
This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human apoLipoprotein J
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of Anti-Apolipoprotein J Antibody. E-LPs attenuate the increased secretion of A Alpha2-macroglobulin induced by NMDA in primary cultures of mixed retinal cells. (C) Conditioned media (HBSS + PBS, n = 5 cell cultures; HBSS + E-LP, n = 5 cell cultures; NMDA + PBS, n = 6 cell cultures; and NMDA + E-LP, n = 6 cell cultures) of the mixed retinal cells were collected 24 hours after treatment with HBSS or 300 uM NMDA treatment with PBS or 300 ng/mL E-LP, and subjected to immunoblotting with antibodies directed against A Alpha2-macroglobulin or apo J. The protein levels were normalized by the corresponding apo J levels. Data are means +/- SD. *, P = 0.0007 for HBSS + PBS versus NMDA + PBS. #, P < 0.0001 for NMDA + PBS versus NMDA + 300 ng/mL E-LP. Data were analyzed with the 1-way ANOVA, Tukey's multiple comparisons test. Figure 3. PMID: 34698771.
Western Blot of Anti-Apolipoprotein J Antibody. E-LPs reduce the expression and secretion of A Alpha2-macroglobulin in primary cultured Mueller glia. (C) Conditioned media C of Mueller glia were collected 24 hours after 0, 100, or 300 ng/mL E-LP treatment (n = 5 cell cultures), and subjected to immunoblotting with antibodies directed against A Alpha2-macroglobulin, apolipoprotein J, apolipoprotein E, or B Beta-actin. The protein levels were normalized by the corresponding apo J C or B Beta-actin D levels. Data are means +/- SD. C *, P = 0.0483 or **, P = 0.0059 for 0 ng/mL E-LP versus 100 or 300 ng/mL E-LP, respectively. D *, P < 0.0001 for 0 ng/mL E-LP versus 100 or 300 ng/mL E-LP. Data were analyzed with the 1-way ANOVA, Tukey's multiple comparisons test. Figure 4. PMID: 34698771.