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Antibodies

Recombinant GST Antibody

Product Sizes
50 ug
£569.00
400-001-MR7-50UG
About this Product
SKU:
400-001-MR7
Additional Names:
recombinant anti-GST antibody, Glutathione-S-Transferase antibody
Application:
Dot Blot, Western Blot
Buffer:
1X PBS
CE/IVD:
RUO
translate.label.attr.clone:
GST13
Clonality:
Recombinant Monoclonal
Concentration:
0.5 mg/ml
Extra Details:
Rockland produces a wide range of GST antibodies in our laboratories. Select GST antibodies from several monoclonal and/or polyclonal GST antibodies listed below. Select appropriate GST antibodies for your research by isotype, epitope, applications and species reactivity. GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors. GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer. A single-domain antibody (sdAb) is a small antibody fragment consisting of the monomeric variable domain derived from camelid heavy chain-only immunoglobulins naturally found in llamas, alpacas and camels. Also known as VHH antibodies, these are the smallest functional antigen-binding fragment that occurs in nature (12 - 14 kDa) and are now being used in biotechnology as a novel antibody scaffold. The small size of the VHH single domain antibody makes it very attractive for use in diagnostic imaging and potentially therapeutic applications.
Formulation:
1X PBS, pH 7.4
Physical State:
Liquid
Reactivities:
Label/Epitope-tag
Shipping Conditions:
Dry Ice
Specificity:
Anti-GST (VHH) Antibody is a recombinant antibody. The clone was isolated from a library prepared from a hyper-immunized llama host and purified by affinity chromatography from bacterial culture. Expect reactivity against GST-labeled probes and proteins.
Storage Conditions:
2-8[o]C Aliquot. Avoid freeze/thaw cycles. Store undiluted., -20[o]C Aliquot. Avoid freeze/thaw cycles. Store undiluted.
Supplier:
Rockland Inc
Type:
Antibody: Recombinant Antibodies
Dot Blot of Anti-GST (VHH) Single Domain Antibody. Row A: Recombinant GST protein (p/n 000-001-200). Amount Dotted: (1) 200, (2) 100, (3) 50, (4) 25, (5) 12.5ng. Primary Antibody: Anti-GST (VhH) 1.0ug/mL for 1.5hr at RT. Secondary Antibody: Mouse Anti-6X His Tag Peroxidase (p/n 200-303-382) at 1:500 for 30 mins at RT. Block: BlockOut Buffer (p/n MB-073).
Western Blot of Anti-GST Single Domain Antibody. Lane 1: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 2: Recombinant GST (p/n 000-001-200) (0.2ug) [+]. Lane 3: HEK293T WCL (p/n W09-001-GX5) / rec. GST (p/n 000-001-200) (10ug/0.2ug) [+]. Lane 4: HEK293T Whole Cell Lysate (p/n W09-001-GX5) (10ug) [-]. Lane 5: E.coli Host Cell Protein (HCP) Control Protein (p/n 000-001-J08) (10ug) [-]. Primary Antibody: Anti-GST (VhH) at 5.0ug/mL overnight at 2-8°C. Secondary Antibody: Mouse Anti-6X His Tag Peroxidase (p/n 200-303-382) at 1:3,000 for 30 mins at RT. Block: BlockOut Buffer (p/n MB-073). Exposure: 60 sec. Predicted MW: ~25.5kDa. Observed MW: ~25.5kDa. Unspecific Bands: ~70kDa minor background due to unspecific cross-reactivity of anti-6X His ab with unknown proteins of HEK293T lysate.