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Antibodies

IL-6 Antibody

Product Sizes
100 ug
£525.00
210-401-310-100UG
About this Product
SKU:
210-401-310
Additional Names:
Il6|rabbit anti-IL-6 antibody, rabbit anti-Interleukin-6 antibody, Interleukin6 cytokine, IL6, B-cell stimulatory factor 2, BSF-2, Interferon beta-2, IFN-beta-2, Hybridoma growth factor, CTL differentiation factor, CDF, Interleukin HP-1
Application:
ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Interleukin (IL)-6, also know as BCDG, BCGF and BSF-2, is an important proinflammatory and immunoregulatory cytokine expressed by various cells. Interleukin-6 has been shown to inhibit the growth of early stage and to promote the proliferation of advanced stage melanoma cells in vitro. Anti-IL-6 antibody is ideal for investigators involved in Cancer, Neuroscience and Immunology research.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-IL-6 is an IgG fraction antibody prepared from rabbit antiserum after repeated immunizations with recombinant mouse IL-6 protein produced in E.coli.
Isotype:
IgG
Physical State:
Lyophilized
Reactivities:
Mouse
Shipping Conditions:
Ambient
Specificity:
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for mouse IL-6 protein. A BLAST analysis was used to suggest cross-reactivity with IL-6 from mouse sources based on 100% homolog
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Flow Cytometry Results of Rabbit Anti-Mouse IL6 Antibody in mouse Raw 264.7 cell line. The orange histogram represents the Raw 264.7 murine cells that were activated with 100ng/mL LPS for 24 hours. The green histogram are untreated Raw 264.7 cells. These two populations were intracellularly stained for 30 minutes at 4°C in 1x BD Perm/Wash™ buffer. The primary stain was a 1:30 dilution of the Anti-Mouse IL-6 (RABBIT) Polyclonal Antibody (p/n 210-401-310, Lot#45055 [stock conc 1mg/mL]) and the secondary stain was the Anti-RABBIT IgG (H&L) (GOAT) Antibody Fluorescein Conjugated (p/n 611-1202 [1:400 dilution of 2mg/mL]). The secondary stain was for 30 minutes at 4°C and was kept protected from light. The blue histogram is the Raw 264.7 murine cells that were untreated and only stained with the secondary antibody. The red histogram is the untreated Raw 264.7 murine cells that were not stained. Prior to staining, the cells for all conditions were permeabilized with BD Fixation/Permeabilization ™ solution for 20 minutes at 4°C. All washes and stains were performed in the BD 1x Perm/Wash™ buffer.
Flow Cytometry Results of Rabbit Anti-Mouse IL6 Antibody in human THP-1 cell line. The orange histogram represents the THP-1 cells that were activated with 100ng/mL LPS for 24 hours. The green histogram are untreated THP-1 cells. These two populations were intracellularly stained for 30 minutes at 4°C in 1x BD Perm/Wash™ buffer. The primary stain was a 1:100 dilution of the Anti-Mouse IL-6 (RABBIT) Polyclonal Antibody (p/n 210-401-310, Lot# 45055 [stock conc 1mg/mL]) and the secondary stain was the Anti-RABBIT IgG (H&L) (GOAT) Antibody Fluorescein Conjugated (p/n 611-1202 [1:400 dilution of 2mg/mL]). The secondary stain was for 30 minutes at 4°C and was kept protected from light. The blue histogram is the THP-1 cells that were untreated and only stained with the secondary antibody. The red histogram is the untreated THP-1 cells that were not stained. Prior to staining, the cells for all conditions were permeabilized with BD Fixation/Permeabilization ™ solution for 20 minutes at 4°C. All washes and stains were performed in the BD 1x Perm/Wash™ buffer.
Immunohistochemistry with anti-IL-6 antibody showing cytoplasmic IL-6 staining in mouse pancreas exocrine and endocrine sections with islets of Langerhans at 10x and 20x (B & C). Staining was performed on Leica Bond system using the standard protocol. Formalin fixed/paraffin embedded tissue sections were subjected to antigen retrieval with E1 (Leica Microsystems) retrieval solution for 20 min and then incubated with rabbit anti-mouse IL-6 antibody at 1:50 dilution for 60 minutes. Biotinylated Anti-rabbit secondary antibody was used at 1:200 dilution to detect primary antibody. The reaction was developed using streptavidin-HRP conjugated compact polymer system and visualized with chromogen substrate, 3'3-diamino-benzidine substrate (DAB). The sections were then counterstained with hematoxylin to detect cell nuclei.
Immunohistochemistry with anti-IL-6 antibody showing positivity of islets of Langerhans (brown staining) and cytoplasmic staining in mouse pancreas at 10x and 20x (B & C). Staining was performed on Leica Bond system using the standard protocol. Formalin fixed/paraffin embedded tissue sections were subjected to antigen retrieval with E1 (Leica Microsystems) retrieval solution for 20 min and then incubated with rabbit anti-mouse IL-6 antibody at 1:50 dilution for 60 minutes. Biotinylated Anti-rabbit secondary antibody was used at 1:200 dilution to detect primary antibody. The reaction was developed using streptavidin-HRP conjugated compact polymer system and visualized with chromogen substrate, 3'3-diamino-benzidine substrate (DAB). The sections were then counterstained with hematoxylin to detect cell nuclei.
Anti-mouse IL-6 antibody in western blot shows detection of recombinant mouse IL-6 raised in E.coli. Recombinant truncated protein (0.1 ug, 21.7 kDa) was loaded on to an SDS-PAGE gel, and after separation, transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Rockland's Anti-Mouse IL-6 antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody Dylight™ 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody (p/n 611-143-122) diluted 1:20,000 in blocking buffer (p/n MB-070) for 30 min at RT. Data was collected using Bio-Rad VersaDoc® 4000 MP imaging system.