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Antibodies

Transferrin Antibody Fluorescein Conjugated

Product Sizes
100 ug
209-4234-0100-100UG
About this Product
SKU:
209-4234-0100
Additional Names:
rabbit anti-Transferrin Antibody, Apotransferrin antibody, Beta 1 metal binding globulin antibody, DKFZp781D0156 antibody, PRO1400 antibody, PRO1557 antibody, PRO2086 antibody, Serotransferrin precursor antibody, Siderophilin antibody, TF antibody|TF
Application:
Dot Blot, ELISA, FLISA, Immunofluorescence, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
FITC
Concentration:
1 mg/ml
Extra Details:
Human transferrin is encoded by the TF gene and is an iron-binding blood plasma glycoprotein that controls the level of free iron in biological fluids. Human transferrin binds iron very tightly but reversibly. Human transferrin is the most important iron pool in mammals. Human transferrin has a molecular weight of around 80 kDa and contains 2 specific high-affinity Fe(III) binding sites. The affinity of Human transferrin for Fe(III) is extremely high but decreases progressively with decreasing pH below neutrality. Human Transferrin also plays a role in the immune system, creating environments low in iron for which many pathogenic bacteria are unable to thrive.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Transferrin (Human Serum)
Isotype:
IgG
Physical State:
Lyophilized
Reactivities:
Human
Shipping Conditions:
Ambient
Specificity:
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum and purified and partially purified Transfe
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles., -20[o]C/-70[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
FluoroFinder:
Rockland Rabbit anti-Transferrin (109-4134 lot 3033, green), Goat-anti-Alpha-1-Anti-Trypsin (100-101-147 lot 5842), and Mouse-a-GST (200-301-200 lot 24882) were used in a multiplex system to detect target proteins under reducing (R) conditions (+4% BME) in albumin depleted human serum with 320 ng of added GST. Sample was run by SDS-PAGE, transferred to 0.2 um PVDF using the BioRad Trans-Blot Turbo and blocked in 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4°C. Membrane was probed with three primary antibodies at 1:1000 dilution (in MB-070 over night at 4°C). Detection shown was using DyLight549 Donkey anti-Rabbit IgG (611-742-127 lot 21100, shown as green) DyLight 488 Donkey anti-Mouse IgG (610-741-124 lot 21095, shown as blue), and DyLight 649 Donkey anti-Goat IgG (605-743-125 lot 20834, shown as red) at 1:10000 (in MB-070 30 min RT). Blots were washed, rinsed in methanol, dried and Images were collected using the BioRad VersaDoc System.
Rockland primary and Dylight conjugated secondary antibodies were used to detect: Human transferrin (1° 109-4134, green 2° 611-743-127); Alpha 1 anti trypsin (1° 100-101-147, red 2° 605-742-125); and Human IgG (1° 109-3102, Blue 2° 610-741-124 in a multiplex fluorescent western blot of human serum. Each primary antibody was diluted to 1:1000 in Blocking Buffer for Fluorescent Western Blotting - MB-070 and incubated for 2 hrs at RT. Blot was 3X in TTBS, 1X in TBS and probed with secondary antibodies diluted 1:10000) in MB-070 and incubated ~ 1hr at 4 degrees. After wash 2X in TTBS and 2X in TBS, blot was rinsed 2X in MeOH, dried and imaged using the Biorad VersaDoc4000.