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Antibodies

CD3e Fluorescein Antibody

Product Sizes
100 ug
200-B02-M69-100UG
About this Product
SKU:
200-B02-M69
Additional Names:
Cd3e|T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e
Application:
Flow Cytometry
Buffer:
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
translate.label.attr.clone:
145-2c11
Clonality:
Monoclonal
Conjugate:
FITC
Concentration:
0.5 mg/ml
Extra Details:
CD3 is a multi-subunit complex consisting of 4 different invariable membrane proteins, CD3E, CD3D, CD3G and CD3-omega. These CD3 proteins are expressed on the surface of T-cells and Thymocytes during thymocyte development. They are non-covalently associated with the TCR heterodimer and form the functional TCR-CD3 antigen receptor complex of the T-lymphocyte. CD3 expression is significant during Intrathymic T-lymphocyte maturation, TCR signaling and Thymocyte differentiation. Stimulation of TCR-CD3 complex results in activation of various protein tyrosine kinases including ZAP-70 affecting multiple cellular responses including T-cell proliferation, clonal expansion, induction of tolerance and apoptosis. Mutations in the sub-unit chains of CD3 have been associated with various immunodeficiency syndromes including SCID.
Formulation:
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Hamster
Immunogen:
Anti-CD3 Antibody (Monoclonal) was produced by repeated immunizations with H-2Kb specific cytotoxic T lymphocyte clone BM10-37.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Mouse
Shipping Conditions:
Blue Ice
Specificity:
Fluorescein conjugated CD3 Monoclonal Antibody was Protein G Purified from tissue culture supernatant and is directed against mouse CD3. Reactivity is observed against mouse CD3. Cross reactivity with CD3 from other sources has not been tested.
Storage Conditions:
2-8[o]C diluted. Do not freeze. Protect from light.
Supplier:
Rockland Inc
Type:
Antibody: Monoclonal Antibody
Cell Surface Flow Cytometry of Hamster anti-MOUSE CD3 antibody Fluorescein conjugated. Cells: 10^6 BALB/c mouse splenocytes. Stimulation: none. Antibody: (GREEN) isotype control antibody; (RED) Fluorescein Anti-CD3 Hamster secondary antibody using 0.25 ug.
Flow Cytometry of Hamster anti-MOUSE CD3e antibody Fluorescein conjugated. Cells: 10^6 C57Bl/6 splenocytes. Stimulation: none. Antibody: (GRAY) 0.5ug FITC Hamster IgG isotype control antibody; (BLUE) Fluorescein Anti-CD3 Hamster secondary antibody using 0.5 ug.
Flow Cytometry of Hamster anti-MOUSE CD3 antibody Fluorescein conjugated. Cells: 10^6 C57Bl/6 splenocytes. Stimulation: none. Antibody: (GRAY) FITC Hamster IgG isotype control antibody; (BLUE) 0.5ug Rockland Fluorescein Anti-CD3 Hamster secondary antibody, (RED) Fluorescein Anti-CD3 Hamster control antibody.