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Antibodies

Beta Galactosidase Antibody

Product Sizes
100 ug
200-901-036-100UG
About this Product
SKU:
200-901-036
Additional Names:
chicken anti-Beta Galactosidase Antibody, chicken anti-Beta Gal Antibody, Beta Galactosidase [E.coli], BGAL, beta-galactosidase, beta-gal|lacZ
Application:
Dot Blot, ELISA, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Anti Beta Galactosidase Antibody recognizes the enzyme beta galactosidase, or B Beta-galactosidase, that is a component of assays used frequently in genetics, molecular biology (see X-gal) for a blue white screen, and other life sciences. IPTG induces production of B Beta-galactosidase by binding and inhibiting the lac repressor. Since it is highly expressed and accumulated in lysosomes in senescent cells, it is used as a senescence biomarker both in vivo and in vitro in qualitative and quantitative assays, despite its limitations. Anti-beta Galactosidase Antibody is ideal for investigators involved in enzyme research.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Chicken/Avian
Immunogen:
This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with full length native Beta Galactosidase isolated from E.coli
Isotype:
IgY
Physical State:
Liquid
Reactivities:
Label/Epitope-tag
Shipping Conditions:
Dry Ice
Specificity:
This Anti-Beta Galactosidase Antibody is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Beta Galactosidase [E.coli] and
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot results of Chicken Anti-Beta Galactosidase Antibody. Lane 1: Opal Prestained Molecular Weight Ladder (p/n MB-210-0500). Lane 2: Beta Galactosidase. Load: 50ng. Primary Antibody: Rabbit Anti-Beta Galactosidase at 1ug/mL overnight at 4°C. Secondary Antibody: Goat anti-Chicken HRP (p/n 603-103-126) at 1:40,000 for 30min at RT. Blocking: BlockOut (p/n MB-073) for 30 min at RT.
Dot Blot of Chicken Anti-Beta Galactosidase Antibody. Dilutions of Beta Gal: (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.7ng, and (5) 1.23ng. Top Blot incubated with Primary Antibody: 1ug/mL of Anti-Beta Galactosidase for 1 hour at room temp. Bottom Blot incubated with Secondary Only: Goat anti-Chicken Peroxidase conjugated (p/n 603-103-126) 1:40,000 for 30 min at room temp. Blocking Buffer: BlockOut Universal Blocker (p/n MB-073). Exposure 1 sec.