Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

Adenosine Deaminase Antibody Peroxidase Conjugated

Product Sizes
100 ug
200-403-140-0100-100UG
About this Product
SKU:
200-403-140-0100
Additional Names:
ADA|rabbit anti-Adenosine Deaminase Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Adenosine Deaminase Antibody, Adenosine deaminase, Adenosine aminohydrolase
Application:
ELISA, Immunoprecipitation, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
HRP
Concentration:
1 mg/ml
Extra Details:
Adenosine deaminase (adenosine aminhydrolase, or ADA) is an enzyme involved in purine metabolism. It is needed for the breakdown of adenosine from food and for the turnover of nucleic acids in tissues. ADA irreversibly deaminates adenosine, converting it to the related nucleoside inosine by the substitution of the amino group for a hydroxyl group. ADA irreversibly deaminates adenosine, converting it to the related nucleoside inosine by the substitution of the amino group for a hydroxyl group. Inosine can then be deribosylated by another enzyme called purine nucleoside phosphorylase (PNP), converting it to hypoxanthine.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Adenosine Deaminase [Calf Spleen] ADA
Isotype:
IgG
Physical State:
Lyophilized
Reactivities:
Bovine
Shipping Conditions:
Ambient
Specificity:
Anti-Adenosine deaminase Peroxidase antibody has been tested by ELISA and western blot and is assayed against 1.0 ug of Adenosine Deaminase [Calf Spleen] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is s
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of Rabbit anti-Adenosine Deaminase Antibody Peroxidase Conjugated. Lane 1: Adenosine Deaminase (Calf Spleen). Load: 50 ng per lane. Primary antibody: Rabbit anti-Adenosine Deaminase Antibody Peroxidase Conjugated at 1:1,000 overnight at 4°C. Secondary antibody: n/a. Block: MB-070 for 30 min at RT. Predicted/Observed size: 41 kDa, 45 kDa for Adenosine Deaminase (Calf Spleen).
Rockland biotin conjugated anti adenosine deaminase (200-406-140) was used to detect adenosine deaminase in mouse pancreas lysate (Left, Lane 1, 30 ul) under reducing conditions. The antibody was also used to detect purified Adenosine Deaminase (right, Lane 2), and endogenous Adenosine Deaminase in whole cell lysate from Jurkat and Raji cells (1:1 mixture, lane 3,) as well as Mouse Pancreas and Liver (1:1 mixture, lane 4). Lysates were run on 4-20% gel 140V under reducing conditions, transferred for 30 minutes at 100 V and blocked with 3% Fish Gel (left) or 3% BSA. Blot was incubated with 200-406-140 lot 5628 (1:5K in TBS, ON 4°C), washed 3X in TBS and incubated for 30 minutes with Dylight 488 conjugated Streptavidin (S000-41 lot 20833 1:5K in MB-070). Blot was imaged with the Biorad VersaDoc imaging system.