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Antibodies

VHH Antibody

Product Sizes
25 ul
£257.00
200-401-GM6S-25UL
About this Product
SKU:
200-401-GM6S
Additional Names:
rabbit anti-VHH Antibody, single-domain antibody (sdAb), camelid VHH antibody
Application:
ELISA, Immunofluorescence, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1.3 mg/ml
Extra Details:
Rabbit Anti-VHH antibody can be used to detect single domain VHH antibodies. The single-domain antibody (sdAb) is a small (12 - 14 kDa) antibody fragment that consists of a monomeric variable domain derived from the heavy chain, also called a VHH antibody. These heavy chain only Fab-like domains have activity that is similar to a whole antibody, and they are able to bind to a specific antigens. SdAb's are derived from camelid species that include llamas, alpacas and camels. Camelids produce both classical (containing heavy and light chain fragments) and non-classical antibody structures (containing only a heavy chain). VHH antibodies are the smallest functional antigen-binding fragment that occurs in nature and these are now being used in biotechnology as a novel antibody scaffold. The small size of the VHH single domain antibody makes it very attractive for use in diagnostic imaging and they have potential for therapeutic activity.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a VHH camelid domain protein.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Other Mammal
Shipping Conditions:
Dry Ice
Specificity:
Anti-VHH is directed against the llama VHH protein fragment. The product was purified from monospecific antiserum by protein A chromatography. Anti-VHH antibody detects recombinant VHH proteins, native Llama IgG2 and native Llama IgG3.
Storage Conditions:
-20[o]C diluted. Avoid freeze/thaw cycles., See Manual Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
Western Blot of Rabbit Anti-VHH Antibody detecting VHH. Lane 1: MW Ladder Opal pre-stained (p/n MB-210-0500). Lane 2: VHH. Load: 0.50 ug per lane. Primary Antibody: Anti-VhH Antibody (p/n 200-401-GM6) at 1 ug/mL for overnight at 4°C. Secondary Antibody: Rabbit Secondary HRP Antibody (p/n 611-103-122) at 1:70,000 for 30 min at RT. Block: MB-070 at RT for 30 min. Predicted/Observed size: ~15 kDa.
Western Blot of Rabbit anti-VHH antibody. Lane 1: Ladder (Opal Prestained). Lane 2: VHH protein (p/n 000-001-GM6). Lane 3: Llama IgG1 protein (p/n 025-0140). Lane 4: Llama IgG2 protein (p/n 025-0144). Lane 5: Llama IgG3 protein (p/n 025-0143). Load: 50 ng per lane. Primary antibody: VHH antibody at 1:1000 for overnight at 4°C. Secondary antibody: Gt-a-Rb HRP (611-103-122) rabbit secondary antibody at 1:70,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: expect 15 and 18 kda band in VHH protein and reactivity with Llama IgG isotypes.
Western Blot of Rabbit anti-VHH antibody. Lane 1: MW ladder (opal pre-stained) p/n (MB-210-0500). Lane 2: Llama IgG1 protein (p/n 025-0140). Lane 3: Llama IgG2 protein (p/n 025-0144). Lane 4: Llama IgG3 protein (p/n 025-0143). Lane 5: VhH protein (p/n 000-001-GM6). Load: 50 ng per lane. Primary antibody: VHH antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary HRP antibody (p/n 611-103-122) at 1:40,000 for 45 min at RT. Block: BlockOut (p/n MB-070) overnight at 4°C. Predicted/Observed size: expect 15 and 18 kda band in VHH protein and reactivity with Llama IgG isotypes.
Western Blot of Rabbit anti-VHH Antibody. Lane 1: Hela whole cell lysate spiked with VHH single-domain antibody. Load: 10 ug lysate with 25 ng VHH. Primary antibody: Rabbit anti-VHH Antibody at 1:1,000 o/n at 4°C. Secondary antibody: HRP Gt-a-Rb IgG (p/n 611-103-122) at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 15 kDa, 15 kDa for VHH.