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Antibodies

CRASP-1 Antibody

Product Sizes
100 ug
£525.00
200-401-C18-100UG
About this Product
SKU:
200-401-C18
Additional Names:
CRASP1|rabbit anti-CRASP-1 Antibody, Borrelia burgdorferi CRASP-1, CRASP1, CRASP 1, Complement regulator acquiring protein 1
Application:
ELISA, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
CRASP-1, or Complement Regulator-Acquiring Surface Protein 1, is a multifunctional protein of Lyme disease-causing B. burgdorferi that binds to several human extracellular matrix proteins and plasminogen, including factor H (resulting in inhibition of complement activation in mammals) and Human Bone Morphogenic Protein 2. These interactions may contribute to adhesion, bacterial colonization, and organ tropism and may allow dissemination of B. burgdorferi in the host. B. burgdorferi spirochetes express up to 5 complement regulator-acquiring surface proteins. Multiple copies of sequences analagous to CRASP-1 genes have been detected in Borrelia plasmids. Borrelia species contain a large number of plasmids, of linear and circular, some of which appear to repeat sequences or contain fragments of other genes. These regions may serve as potentially usable information for the survival of Borrelia in its multiple environments during its life cycle. In addition, the sequence for CRASP-1 contains a repeated sequence folded into a stable stem loop structure typical of RNA genes.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
MBP-fusion protein corresponding to Borrelia burgdorferi CRASP-1 protein.
Isotype:
IgG
Physical State:
Lyophilized
Reactivities:
Bacteria
Shipping Conditions:
Ambient
Specificity:
This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Borrelia burgdorferi CRASP-1 protein. A BLAST analysis was used to suggest reactivity with CRASP-1 from B. burgdorferi and B. garinii sources based on 100% homology with the immunizing sequence. Partial cross-reactivity is expected against B. spielmanii, a
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles., -20[o]C/-70[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot results of Rabbit Anti-Crasp-1 Antibody. Lane 1: Crasp 1 protein (p/n 000-001-C18). Lane 2: MBP (p/n 000-001-385). Load: 0.05 uL. Primary Antibody: Rabbit Anti-Crasp-1 Antibody (p/n 200-401-C18) at 1.0mg/mL overnight at 4°C. Secondary Antibody: Goat anti-Rabbit (p/n 611-101-122) at 1:70,000 for 30 min at RT. Blocking: BlockOut Buffer (p/n MB-073) for 30min at RT. Expect: ~63.9kDa.
Western blot showing detection of 0.1 ug of recombinant CRASP-1 protein. Lane 1: Molecular weight markers. Lane 2: MBP-CRASP-1 fusion protein (arrow; expected MW = 69.3 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 um nitrocellulose. After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) overnight at 4°C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit (p/n 611-103-122) secondary antibody was used at 1:40,000 in MB-070 blocking buffer and imaged on the VersaDoc™ MP 4000 imaging system (Bio-Rad).