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Antibodies

OspA Antibody

Product Sizes
25 ul
200-401-C13S-25UL
About this Product
SKU:
200-401-C13S
Additional Names:
ospA|rabbit anti-OspA Antibody, Outer surface protein A, Borrelia burgdorferi OspA, ospA, BB_A15, Plasmid lp54
Application:
ELISA, Immunofluorescence, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1.14 mg/ml
Extra Details:
Outer-Surface Protein A (OspA), a lipoprotein from Borrelia burgdorferi encoded on its Plasmid lp54, is a major component of the spirochete's extracellular matrix. OspA probably serves as a lipid-anchor. The spirochetes migrate from the tick midgut during feeding to its salivary glands and are thus transmitted to the mammal host. This transition may be facilitated by changes in expression of some B. burgdorferi genes. Upon transmission of the spirochete from the Ixodes tick to mammalian host, the transcript level of OspA can change. It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH, and nutrients) and/or in coordination with the course of infection of the mammal host. B. burgdorferi can attach to (and also differentially express antigens in) diverse tissues within the vertebrate host and the tick vector, suggesting that physiological factors other than pH and temperature may play roles in modulating B. burgdorferi gene expression.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
MBP-fusion protein corresponding to Borrelia burgdorferi OspA protein.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Bacteria
Shipping Conditions:
Dry Ice
Specificity:
This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Borrelia burgdorferi OspA protein. A BLAST analysis was used to suggest cross-reactivity with OspA from B. burgdorferi and sources based on 100% homology with the immunizing sequence. Cross-reactivity with OspA or Osp from other sources has not been determ
Storage Conditions:
-20[o]C diluted. Avoid freeze/thaw cycles., See Manual Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of Anti-OspA Antibody. Lane 1: Opal Pre-stained Molecular Weight Marker (p/n MB-210-0500). Lane 2: 10ug HeLa spiked 50ng OspA. Lane 3: 10ug HeLa spiked 20ng OspA. Lane 4: 10ug HeLa spiked 10ng OspA. Lane 5: 10ug HeLa Whole Cell Lysate (p/n W09-000-364). Lane 6: 10ug HeLa spiked 50ng OspB. Lane 7: 10ug HeLa spiked 50ng OspC. Primary Antibody: Anti-OspA at 1:1000 overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n 611-103-122) at 1:70,000 for 30 min at RT. Block: BlockOut Buffer (p/n MB-073). Predicted MW: ~28.1kDa OspA protein, 70.5kDa MBP-OspA fusion. Observed MW: ~30kDa, ~75kDa.
Western blot showing detection of 0.1 ug of recombinant OspA protein. Lane 1: Molecular weight markers. Lane 2: MBP-OspA fusion protein (arrow; expected MW: 70.5 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 um nitrocellulose. After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) overnight at 4°C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit (p/n 611-103-122) secondary antibody was used at 1:40,000 in MB-070 blocking buffer and imaged on the VersaDoc™ MP 4000 imaging system (Bio-Rad).