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Antibodies

Zic-1 Antibody

Product Sizes
25 ul
200-401-159S-25UL
About this Product
SKU:
200-401-159S
Additional Names:
rabbit anti-Zic-1 Antibody, Odd paired homolog Drosophila antibody, Zic 1 antibody, ZIC antibody, Zic family member 1 (odd-paired Drosophila homolog) antibody, Zic family member 1 antibody|Zic1
Application:
Detection/Quantitation, ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Anti Zic-1 Antibody recognizes the product of the Zic-1 gene, that encodes a zinc finger protein which is expressed in the developing or matured central nervous system in a highly restricted manner. Zic-1 is expressed in granule cells that make synaptic contact with Purkinje cells. Clearly Zic-1 is a gene critical to cerebellar pattern formation. The expression of Zic genes is first detected at gastrulation and at neurulation, becomes restricted to the dorsal neural ectoderm and the dorsal paraxial mesoderm. Zic-2 and Zic-3 are highly similar genes, especially in their product's zinc finger motif and by comparison of their genomic organization in that they share common exon-intron boundaries and belong to the same gene family. By comparison in function, Zic-2 is essential for the formation of the brain and Zic-3 is important for right and left axis formation. The Zic-1 gene has been mapped to chromosome 9 in mouse. The 5' flanking region of the Zic-1 gene contains a region-specific enhancer determined to be essential in in vivo and in vitro deletion analysis. The temporal profile of mRNA expression differs for each of the Zic gene products. The Drosophila odd-paired gene is highly homologous to the Zic gene family.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with an 18 aa synthetic peptide from a region near the N-Terminus of mouse Zic-1. This domain is completely conserved in human ZIC-1.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human, Mouse
Shipping Conditions:
Dry Ice
Specificity:
This is an IgG preparation of whole rabbit antiserum purified by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is directed against Zic-1 from mouse. In general, this antibody also detects human Zic-1. Cross-reactivity with other species is likely but has not been det
Storage Conditions:
-20[o]C diluted. Avoid freeze/thaw cycles., See Manual Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western blot. Analysis of Zic1 in mouse cerebellum extract. Protein extracts were prepared from mouse cerebellum between postnatal day 5 (P5) and P18, as indicated above the lanes. ROCKLAND Immunochemical's anti-Zic1 antibody recognizes a single band in all extracts. The positions of the molecular weight markers (in kDa) in the gel are indicated on the left. Personnel communication, C. Kurschner.
A similar time course experiment is shown using mouse cerebellum extracts at various time points. A 10% SDS-PAGE gel was used to separate proteins prior to transfer to nitrocellulose. The membrane was probed with a 1:5,000 dilution of the antibody. The lower minor band may be a breakdown product of Zic1 or it may represent cross reactivity of the detection antibody. HRP conjugated anti-Rabbit IgG (Chemicon) was used at a 1,000 dilution. Personnel communication, K.H. Herzog.