CD3 Allophycocyanin Antibody
Product Sizes
500 ul
£175.00
200-326-N14-500UL
About this Product
- SKU:
- 200-326-N14
- Additional Names:
- CD3E|T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e
- Application:
- Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot
- Buffer:
- 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride
- CE/IVD:
- RUO
- translate.label.attr.clone:
- UCHT1
- Clonality:
- Monoclonal
- Conjugate:
- APC
- Concentration:
- 0.05 mg/ml
- Extra Details:
- The UCHT1 antibody is specific for human CD3e, also known as CD3 epsilon, a 20 kDa subunit of the T cell receptor complex, along with CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor - CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. These interactions promote T cell receptor signaling (T cell activation), inducing cell proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells. The UCHT1 antibody is a widely used phenotypic marker for human T cells. In addition, binding/cross-linking of UCHT1 antibody to CD3e can induce cell activation. A recent publication of the crystal structure of a CD3e- antibody complex provides insight as to the action of commonly used agonist antibodies, as well as specific epitope-binding data for the human CD3 antibodies UCHT1 and OKT3. UCHT1 antibody reacts with both surface-expressed and intracellular CD3e protein, in contrast to an alternative human CD3 clone, HIT3a, which will stain only the extracellular (membrane-expressed) CD3e protein. Also, the UCHT1 antibody is reported to be cross-reactive with chimpanzee and has been used for phenotypic analysis of expression by flow cytometry; however the antibody is reported to be unsuitable for induction of T cell activation in this species.
- Formulation:
- 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- Host:
- Mouse
- Immunogen:
- Anti-CD3 Antibody (Monoclonal) was produced by repeated immunizations with CD3 antigen.
- Isotype:
- IgG1, IgG1, kappa
- Physical State:
- Liquid
- Reactivities:
- Human
- Shipping Conditions:
- Blue Ice
- Specificity:
- Allophycocyanin conjugated CD3 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD3. Reactivity is observed against human and chimpanzee. Cross reactivity with CD3 from other sources has not been tested. Anti-CD3 is conjugated with APC under optimal conditions and the solution is free of unconjugated APC.
- Storage Conditions:
- 2-8[o]C unopened. Protect from light.
- Supplier:
- Rockland Inc
- Type:
- Antibody: Monoclonal Antibody
- FluoroFinder:
- Manufacturer's Data Sheet:https://www.rockland.com/search/?searchString=200-326-N14
