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Antibodies

Calponin Antibody

Product Sizes
100 ug
£546.00
200-301-P26-100UG
About this Product
SKU:
200-301-P26
Additional Names:
Calponin H2, smooth muscle, Neutral calponin|CNN2
Application:
ELISA, Immunohistochemistry, Western Blot
Buffer:
1.2% sodium acetate
CE/IVD:
RUO
translate.label.attr.clone:
CP-93
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
Calponin, a basic 34-kD protein, was first isolated from chicken gizzard and bovine aorta. It is specifically expressed in smooth muscle and binds calmodulin, actin, and tropomyosin. It is able to inhibit the ATPase activity of myosin and is thought to play a role in smooth muscle contraction. Calponin is a smooth muscle specific, actin-, tropomyosin- and calmodulin-binding protein thought to be involved in some way in the regulation or modulation of contraction. Anti-Calponin antibody is suitable for researchers interested in cardiovascular diseases, Calcium Regulation in Cardiac Cells, myometrial pathways, skin cancer research, and down syndrome.
Formulation:
1.2% sodium acetate
Host:
Mouse
Immunogen:
Calponin antibody was produced in mice by repeated immunizations with turkey gizzard calponin.
Isotype:
IgG1
Physical State:
Lyophilized
Reactivities:
Avian, Human
Shipping Conditions:
Ambient
Specificity:
Anti-Calponin antibody was purified from mouse ascites by protein A chromatography. This product reacts with human, chicken, and turkey. Cross reactivity with Calponin from other sources has not been determined.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Antibody: Monoclonal Antibody
Western blot analysis of Calponin/CNN2 using anti-Calponin/CNN2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: chicken heart tissue lysates, Lane 2: chicken heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with affinity purified mouse anti-Calponin/CNN2 antigen monoclonal antibody at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at 1:10,000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Calponin/CNN2 at approximately 33 kDa. The expected band size for Calponin/CNN2 is at 33 kDa.