DNMT1 Antibody
Product Sizes
100 ug
£734.00
200-301-H69-100UG
About this Product
- SKU:
- 200-301-H69
- Additional Names:
- AIM, CXXC9, DNMT, DNA (cytosine-5)-methyltransferase 1, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI|DNMT1
- Application:
- ChIP, ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
- Buffer:
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
- CE/IVD:
- RUO
- translate.label.attr.clone:
- 60B1220.1
- Clonality:
- Monoclonal
- Concentration:
- 1 mg/ml
- Extra Details:
- Anti-Dnmt1 antibody detects human Dnmt1. Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumor suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells. Anti-Dnmt1 antibody is ideal for investigators involved in cancer, cell cycle protein research and epigenetics.
- Formulation:
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- Host:
- Mouse
- Immunogen:
- Dnmt1 Antibody was produced in mice prepared by repeated immunizations with a synthetic peptide corresponding to internal amino acids with Dnmt1 human protein.
- Isotype:
- IgG1, IgG1, kappa
- Physical State:
- Liquid
- Reactivities:
- Human, Mouse
- Shipping Conditions:
- Dry Ice
- Specificity:
- Anti-Dnmt1 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Dnmt1 from human, mouse and Zebrafish based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-Dnmt1 from other sources has not been determined.
- Storage Conditions:
- -20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles., 2-8[o]C diluted. Aliquot. Avoid freeze/thaw cycles.
- Supplier:
- Rockland Inc
- Type:
- Antibody: Monoclonal Antibody
- Manufacturer's Data Sheet:https://www.rockland.com/search/?searchString=200-301-H69
