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Antibodies

Caspase-8 Antibody

Product Sizes
100 ug
200-301-H64-100UG
About this Product
SKU:
200-301-H64
Additional Names:
Casp8|MCH5Caspase-8, CASP-8, CASP8, 3.4.22.61, Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4, FADD-homologous ICE/ced-3-like protease, FADD-like ICE, FLICE, ICE-like apoptotic protease 5, MORT1-associated ced-3 homolog, MACH, Caspase-8 subunit p18, Caspase-8 subunit p10
Application:
Flow Cytometry, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
translate.label.attr.clone:
90A992
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
Anti-Caspase-8 detects human Caspase. Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Caspase-8 (FLICE) forms a direct link between the activation of CD95 and the caspase pathway. Overexpression of Caspase-8 induces apoptosis, which can be blocked by inhibitors specific for the ICE family. Anti-Caspase-8 antibody is ideal for investigators involved in apoptosis research.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Mouse
Immunogen:
Caspase-8 Antibody was produced in mice prepared by repeated immunizations with amino acids at the internal portion of human Caspase-8.
Isotype:
IgG1
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Dry Ice
Specificity:
Anti-Caspase-8 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Anti-Caspase-8 from Chimpanzee, Human, Rhesus Monkey and Mouse based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-Caspase-8 from other sources has not been determined.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Monoclonal Antibody
Immunohistochemistry of mouse Anti-Caspase-8 antibody. Tissue: Human stomach tumor. Fixation: Formalin fixed paraffin embedded. Antigen retrieval: Not required. Primary antibody: Caspase-8 at 5 ug/ml for 1 h at RT (bottom left/right). Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Staining: Top left: Unstained isotype control. Bottom left, right: Caspase-8 is stained brown.
Western Blot of Mouse Anti-Caspase-8 antibody. Lane 1-5: Jurkat cells lysate. Treated with 2 uM of staurosporine for 0, 0.5, 1, 2, 4 hours respectively. Primary antibody: Caspase-8 antibody at 1 ug/ml for overnight at 4°C. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 55.4 kDa for Pro-Caspase-8. Other band(s): p18, p41/p43 cleavage fragments.
Flow Cytometry of Mouse Anti-Caspase-8 antibody. Cells: HeLa cells. Stimulation: none. Primary Antibody: Caspase-8 antibody at 0.1 ug/mL (red) and isotype control (green). Secondary Antibody: Goat anti-mouse IgG-FITC.