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Antibodies

Beta Actin Antibody

Product Sizes
25 ul
200-301-E69S-25UL
About this Product
SKU:
200-301-E69S
Additional Names:
ACTB|mouse anti-beta Actin Antibody, Actin Antibody, Loading Control Antibody, beta actin, B Beta actin, anti-beta actin antibody
Application:
ELISA, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
translate.label.attr.clone:
3H2.E4.E12
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins. Anti-Actin is a loading control antibody and is critical for the correct interpretation of your western blot. Antibodies to loading controls are used to normalize the levels of protein detected by confirming that protein loading is uniform across the gel.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Mouse
Immunogen:
Anti-beta Actin (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with B Beta-Actin peptide near the N-Terminus followed by hybridoma development.
Isotype:
IgG2a, IgG2a, lambda
Physical State:
Liquid
Reactivities:
Human, Mouse
Shipping Conditions:
Dry Ice
Specificity:
Anti-beta Actin was purified from mouse ascites by Protein A chromatography followed by extensive dialysis against the buffer stated above. beta Actin antibody is specific for human and mouse B Beta Actin protein. Expected to cross-react with a wide range of species due to sequence homology. A BLAST analysis was used to suggest that this antibody would react with beta Actin from a wide range of or
Storage Conditions:
-20[o]C diluted. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Monoclonal Antibody
Western Blot of Mouse anti-beta Actin antibody. Marker: Opal Pre-stained ladder (p/n MB-210-0500). Lane 1: HEK293 lysate (p/n W09-000-365). Lane 2: HeLa Lysate (p/n W09-000-364). Lane 3: MCF-7 Lysate (p/n W09-000-360). Lane 4: Jurkat Lysate (p/n W09-000-370). Lane 5: A431 Lysate (p/n W09-000-361). Lane 6: A549 Lysate (p/n W09-001-372). Lane 7: LNCap Lysate (p/n W09-001-GJ9). Lane 8: MOLT-4 Lysate (p/n W09-001-GK2). Lane 9: Raji Lysate (p/n W09-001-368). Lane 10: Ramos Lysate (p/n W09-000-GK4). Lane 11: PC-12 (p/n W12-001-GL9). Load: 10 ug per lane. Primary antibody: beta-Actin antibody at 1:1,000 o/n at 4C. Secondary antibody: Peroxidase mouse secondary (p/n 610-403-C46) at 1:40,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS (p/n MB-082) for 30 min at RT. Predicted/Observed size: 42 kDa for beta-Actin.
Immunohistochemistry of Mouse anti-Beta Actin N-Term antibody. Tissue: human spleen. Fixation: formalin fixed paraffin archival tissues. Antigen retrieval: not required. Primary antibody: Beta Actin N-Term antibody at 2.5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT. Localization: membranous and cytoplasmic. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.
Western Blot of Mouse anti-N-Term Beta Actin Monoclonal Antibody. Lane 1: HeLa Whole Cell Lysate (p/n W09-000-364). Lane 2: NIH/3T3 Whole Cell Lysate (p/n W10-000-358). Load: 10 ug per lane. Primary antibody: N-Term Beta Actin Monoclonal Antibody at 1.13ug/mL overnight at 4°C. Secondary antibody: HRP mouse secondary antibody (p/n 610-403-C46) at 1:40,000 for 30 min at RT. Block: (p/n MB-070) for 30 min at RT. Predicted/Observed size: 42 kDa, 42 kDa for N-Term Beta Actin.