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Antibodies

Mesothelin Antibody

Product Sizes
25 ul
200-301-A88S-25UL
About this Product
SKU:
200-301-A88S
Additional Names:
mouse anti-Mesothelin Antibody, Mesothelian, MN, MB, Pre-pro-megakaryocyte-potentiating factor, CAK1 antigen|MSLN
Application:
ELISA, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
translate.label.attr.clone:
MN-1
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
Mesothelin is a glycosyl-phosphatidyl-inositol-anchored glycoprotein present on the cell surface of various human solid tumors. The mesothelin (MSLN) gene encodes a 71-kDa precursor protein that is processed to a 40-kDa glycosylphosphatidyl-inositol-anchored protein that composes the mature portion and an NH2-terminal 31-kDa fragment called megakaryocyte-potentiating factor that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues, such as mesothelium, but aberrantly over expressed in mesotheliomas, ovarian, and pancreatic cancers. The biological functions of mesothelin remain elusive. A recent study showed that mesothelin binds to MUC16/CA125, and that this interaction mediates cell adhesion, suggesting that there may be an important role for MUC16/CA125 and mesothelin in the metastatic spread of ovarian cancer.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Mouse
Immunogen:
This antibody was produced in mesothelin-deficient mice by immunizations with plasmid cDNA encoding human MSLN full length protein followed by a single boost of a recombinant human mesothelin-Fc fusion protein.
Isotype:
IgG2a
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Dry Ice
Specificity:
This antibody is directed against human mesothelin protein. This product was purified from tissue culture supernatant fluid by Protein A chromatography. Cross reactivity with homologues from other sources has not been tested.
Storage Conditions:
-20[o]C diluted. Avoid freeze/thaw cycles., See Manual Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Monoclonal Antibody
Immunohistochemistry of Mouse anti-Mesothelin antibody. Tissue: human tonsil. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: anti-Mesothelin antibody at 15 ug/mL for 1 h at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Staining: Mesothelin as precipitated red signal with hematoxylin purple nuclear counterstain.
Flow Cytometry Results of Anti-Mesothelin (MOUSE) Monoclonal Antibody. The green histogram shows NCI-H226 cells and blue histogram shows MCF-7 cells. Both cell lines are stained with a 1:800 dilution Anti-Mesothelin (MOUSE) Monoclonal Antibody. The secondary antibody use was Anti-Mouse IgG (H&L) (GOAT) Antibody DyLight™ 488 Conjugated (p/n 610-141-002, lot#43322) at the 1:400 dilution.
Western Blot of Mouse Anti-Mesothelin Antibody. Lane 1: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 2: HeLa [10ug] + Mesothelin-Fc [0.1ug]. Lane 3: HeLa [10ug] + Mesothelin-Fc [0.05ug]. Lane 4: HeLa [10ug] + Mesothelin-Fc [0.02ug]. Lane 5: HeLa Whole Cell Lysate (p/n W09-000-364). Primary Antibody: Anti-Mesothelin at 1ug/mL overnight at 2-8°C. Secondary Antibody: Rabbit Anti-Mouse IgG HRP conjugated (p/n 610-4302) at 1:40,000 for 30 mins at RT. Block: BlockOut Buffer (p/n MB-073) 30 mins at RT. Exposure: 15 sec. Predicted MW: 40kDa Mesothelin + Fc region 30kDa. Observed MW: ~70-75kDa.
Immunohistochemistry using Rockland's anti-mesothelin antibody to react with two epitopes on mesothelin in PEFF human mesothelioma tissue sections treated by antigen retrieval methods. Anti-mesothelin primary antibodies were used at 10 ug/mL to label these sections as follows: C, MAb MB; and D, MAb MN followed by goat anti-mouse IgG conjugated to horseradish peroxidase at 25 ug/mL in 1% BSA/PBS for 30 minutes. (magnification, x200; bar, 50 um). Reprinted with permission from Clin.Cancer Res. 11(16):5840-6.
Western blotting using Rockland's anti-mesothelin antibodies to detect mesothelin-Fc at 100 ng (lane 1), 25 ng (lane 2), 6 ng (lane 3), 2 ng (lane 4) and 0.4 ng (lane 5). Lane 6 contains 50 ng of CDC25-Fc. Proteins were separated on 4-20% gradient gel by SDS-PAGE followed by transfer to PVDF membrane. Primary antibody was used at 1 ug/ml followed by reaction with ALP goat anti-mouse IgG and BCIP/NBT substrate. Reprinted with permission from Clin.Cancer Res. 11(16):5840-6.