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Antibodies

Alpha-Tubulin Antibody

Product Sizes
25 ul
200-301-880S-25UL
About this Product
SKU:
200-301-880S
Additional Names:
mouse anti-Alpha-Tubulin Antibody, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, TUBA1B, tubulin loading control|TUBA1B
Application:
Detection/Quantitation, ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
translate.label.attr.clone:
17H11.F10
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. This antibody makes an excellent loading control.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Mouse
Immunogen:
Anti-Tubulin Loading Control Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues near the C terminal end of human alpha tubulin protein.
Isotype:
IgG1, IgG1, kappa
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Dry Ice
Specificity:
Anti-Tubulin Loading Control Antibody was purified by Protein A chromatography. This Loading Control antibody is directed against alpha tubulin. A BLAST analysis was used to suggest antibody reactivity with alpha tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with
Storage Conditions:
-20[o]C diluted. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Monoclonal Antibody
Immunofluorescence microscopy of Anti-tubulin in U-87 MG cells using FITC-conjugated Fluorescent TrueBlot® anti-mouse IgG (p/n 18-0217-32) for detection. U87-MG cells were fixed with 100% methanol, permeabilized with 0.3% Triton X-100, blocked with 5% rat serum/0.3 Triton X-100 for 1 hr, then incubated with 15 ug/mL of anti-a-tubulin primary antibody (p/n 200-301-880) at 4°C overnight. Following 3 washes in 1X PBS for 5min each, 5ug/mL of Fluorescent TrueBlot® anti-mouse IgG Fluorescein (p/n 18-0217-32) was added and allowed to incubate for 1hr at room temperature. 5 ug/mL of Fluorescent TrueBlot® anti-mouse IgG FITC was added and allowed to incubate for 1hr at room temperature. Nucleus was counterstained with DAPI present in mounting medium. The predicted main localization is microtubules. Image taken at 63X magnification. Merged a-tubulin (green)/DAPI (blue) image shown.
Immunofluorescence microscopy of A Alpha-tubulin in U-87 MG cells using FITC-conjugated Fluorescent TrueBlot® anti-mouse IgG (p/n 18-0217-32) for detection. U87-MG cells were fixed with 100% methanol, blocked (5% rat serum/0.3% Triton X-100) for 1hr, then incubated with 15ug/mL of anti-alpha-tubulin primary antibody (p/n 200-301-880) at 4°C overnight. Following 3 washes in 1X PBS for 5min each, 5ug/mL of Fluorescent TrueBlot® anti-mouse IgG Fluorescein was added and allowed to incubate for 1hr at room temperature. 5ug/mL of Fluorescent TrueBlot® anti-mouse IgG FITC was added and allowed to incubate for 1hr at room temperature. Nucleus was counterstained with DAPI present in mounting medium. The predicted main localization is microtubules. Image taken at 63X magnification. (a) Merged A Alpha-tubulin (green)/DAPI (blue) image shown (b) secondary only.
Immunofluorescence microscopy of A Alpha-tubulin in HeLa cells using DyLight™ 680-conjugated Fluorescent TrueBlot® anti-mouse IgG (p/n 18-4417-32) for detection. HeLa cells were fixed with 100% methanol, blocked (5% rat serum/0.3% Triton X-100 in 1X PBS) for 1hr, then incubated with 15ug/mL of anti-alpha-tubulin primary antibody (p/n 200-301-880) at 4°C overnight. Following 3 washes in 1X PBS for 5 min each, 5ug/mL of Fluorescent TrueBlot® anti-mouse IgG DyLight™ 680 was added and allowed to incubate for 1hr at room temperature. Nuclei were counterstained with DAPI present in mounting medium. The predicted main localization is microtubules. Image taken at 63X magnification. (a) Merged a-tubulin (red)/DAPI (blue) image shown. (b) secondary antibody only.
Immunohistochemistry of Mouse anti-Alpha-Tubulin antibody. Tissue: human colon. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: anti-Alpha-Tubulin antibody at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Staining: Alpha-Tubulin as precipitated red signal with hematoxylin purple nuclear counterstain.
Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody. Immunofluorescence image showing MCF-7 cell staining of Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody - (p/n 200-301-880) in green and staining of Anti-Gli-3 (RABBIT) Antibody - (p/n 600-401-694) in red.