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Antibodies

Cdc2 p34 Antibody

Product Sizes
100 ug
200-301-160-100UG
About this Product
SKU:
200-301-160
Additional Names:
CDK1|mouse anti-Cdc2 antibody, mouse anti-p34 antibody, Cell Division Cycle 2 Protein antibody, Cyclin Dependent Kinase 1 antibody, p34 Cdk1 antibody, p34 protein kinase antibody, CDC28A, CDKN1, P34CDC2
Application:
ELISA, Immunoprecipitation, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.5 M Sodium Chloride
CE/IVD:
RUO
translate.label.attr.clone:
POH-1
Clonality:
Monoclonal
Concentration:
1.023 mg/ml
Extra Details:
p34 cdc2 is a serine-threonine protein kinase of 34,000 daltons that complexes with cyclin to form maturation promoting factor (MPF). The inactive form of the protein is phosphorylated at threonine (T) and tyrosine (Y) residues. In humans the phosphorylation appears to be performed by p60src. The active form of the protein is dephosphorylated and it functions by phosphorylating a number of proteins. The phosphorylation activity is coupled to the entry into the M-phase of the cell. p34 cdc2 protein must be associated with a normal cyclin protein for the M-phase to be completed normally. Association with deletion mutants of cyclin halts the M-phase before it is completed.
Formulation:
0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
Host:
Mouse
Immunogen:
This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p34 cdc2 fusion protein.
Isotype:
IgG2a, IgG2a, kappa
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Dry Ice
Specificity:
This protein A purified mouse monoclonal antibody reacts specifically with p34 cdc2 in human tissues and cell lines. This antibody is not cross reactive with other cyclin dependent kinases. Cross reactivity with p34 cdc2 from other sources, especially mouse and rat will occur. This reagent has broad interspecies reactivity.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Monoclonal Antibody
Mab anti-Human p34cdc2 antibody (clone POH-1) is shown to detect human p34cdc2 by western blot. Detection occurs after 10 ug of a HeLa whole cell lysate is loaded per lane. The blot was incubated with a 1:1,000 dilution of Mab anti-Human p34cdc2 at room temperature for 30 min followed by detection using IRDye™800 labeled Goat-a-Mouse IgG [H&L] (610-132-121) diluted 1:5,000. A doublet band corresponding to human p34cdc2 is detected at ~34 kDa when compared with known molecular weight standards (not shown). The antibody may be used to detect endogenous human p34cdc2. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Mab anti-Human p34cdc2 antibody was used to detect human p34cdc2 by western blot in untreated (control) and drug treated (10 uM genistein) lysates of MCF-7 cells. Very strong detection occurs using a 1:1,000 dilution. Personnel Communication, Xiao He Yang, University of Oklahoma Health Sciences Center.
Western Blot of Mouse anti-CDC2 (p34) antibody. Marker: Opal Pre-stained ladder (p/n MB-210-0500). Lane 1: HEK293 lysate (p/n W09-000-365). Lane 2: HeLa Lysate (p/n W09-000-363). Lane 3: MCF-7 Lysate (p/n W09-000-360). Lane 4: Jurkat Lysate (p/n W09-000-370). Lane 5: A549 Lysate (p/n W09-001-372). Lane 6: HL-60 Lysate (p/n W09-001-GL3). Lane 7: Raji Lsyate (p/n W09-001-368). Lane 8: NIH/3T3 Lysate (p/n W10-000-358). Load: 35 ug per lane. Primary antibody: CDC2 (p34) antibody at 1:5,000 for overnight at 4°C. Secondary antibody: Peroxidase mouse secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 34 kDa for CDC2 (p34).