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Antibodies

Biotin Monoclonal Antibody

Product Sizes
25 ul
£254.00
200-301-098S-25UL
About this Product
SKU:
200-301-098S
Additional Names:
mouse anti-Biotin Monoclonal Antibody, Anti-Biotin, Vitamin B4 antibody
Application:
ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Lateral Flow Assay, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
translate.label.attr.clone:
4C7.D5
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
Localization of biotinylated probes with avidin produces may produce high background levels. Anti-biotin antibodies may be substituted for avidin to decrease non-specific binding.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Mouse
Immunogen:
Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH)
Isotype:
IgG2a, IgG2a, lambda
Physical State:
Liquid
Reactivities:
Label/Epitope-tag
Shipping Conditions:
Dry Ice
Specificity:
Biotin Antibody was prepared from mouse ascites by immunoaffinity chromatography using Biotin coupled to sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-mouse serum, Biotin conjugated IgG and Biotin conjugated Bovine Serum Albumin.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Antibody: Monoclonal Antibody
Immunofluorescence Microscopy of Mouse Anti-Biotin antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: Biotinylated anti-transferrin receptor antibody. Secondary antibody: CF™488A anti-biotin secondary antibody at 2ug/mL for 45 min at RT. Localization/Staining: plasma membrane and endosome.
Immunofluorescence Microscopy of Mouse Anti-Biotin antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Gain: 800. Primary antibody: Biotinylated anti-transferrin receptor antibody. Secondary antibody: CF™488A anti-biotin secondary antibody at 1ug/mL for 45 min at RT. Localization/Staining: plasma membrane and endosome.
Immunofluorescence Microscopy of Mouse Anti-Biotin antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Gain: 800. Primary antibody: Biotinylated anti-transferrin receptor antibody. Secondary antibody: CF™488A anti-biotin secondary antibody at 2ug/mL for 45 min at RT. Localization/Staining: plasma membrane and endosome.
Western Blot of Mouse Anti-Biotin Monoclonal Antibody. Lane 1: Opal PreStained Molecular Weight Marker (p/n MB-210-0500). Lane 2: Bovine Albumin (BSA) (p/n 001-0133) [20ng]. Lane 3: Bovine Albumin-Biotin conjugated (p/n 001-0633) [20ng). Primary Antibody: Anti-Biotin at 1.0ug/mL overnight at 2-8°C. Secondary Antibody: Rabbit Anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody Peroxidase Conjugated (p/n 610-403-C46) at 1:40,000 for 30mins at RT. Blocking Buffer: BlockOut Buffer (p/n MB-073) for 1hr at RT. Predicted MW: 75kDa. Exposure: 2sec.