Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

Pepsin Antibody

Product Sizes
100 ug
£525.00
200-1176-0100-100UG
About this Product
SKU:
200-1176-0100
Additional Names:
goat anti-Pepsin Antibody, Pepsin A antibody, Pepsin a precursor antibody, Pepsin antibody, Pepsinogen 1 second locus antibody, Pepsinogen 3 group 1 antibody, Pepsinogen a3 antibody|PGA
Application:
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Pepsin antibody detects Pepsin protein. Pepsin is an enzyme whose pepsinogen is released by the chief cells in the stomach and degrades food proteins into peptides. Pepsin belongs to the peptidase A1 family and is one of three principal proteolytic enzymes in the digestive system. Pepsin is most efficient in cleaving peptide bonds between hydrophobic and preferably aromatic amino acids such as phenylalanine, tryptophan, and tyrosine at positions P1 and P1'. Anti-Pepsin Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Goat
Immunogen:
Anti-Pepsin Antibody was produced by repeated immunizations with pork Pepsin protein isolated from swine stomach.
Isotype:
IgG
Physical State:
Lyophilized
Reactivities:
Porcine
Shipping Conditions:
Ambient
Specificity:
Anti-PEPSIN is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Pepsin [Porcine Stoma
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles., -20[o]C/-70[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Immunohistochemistry of goat anti-pepsin antibody at 1:100.
Western Blot of Anti-Pepsin Antibody. Lane 1: Opal Prestained Molecular Weight Ladder (p/n MB-210-0500). Lane 2: HeLa Lysate (10ug) + Pepsin (0.1ug) [+]. Lane 3: HeLa Lysate (10ug) + Pepsin (0.05ug) [+]. Lane 4: HeLa Lysate (10ug) + Pepsin (0.02ug) [+]. Lane 5: HeLa Lysate (p/n W09-000-364) (10ug) [-]. Primary Antibody: Anti-Pepsin at 1ug/mL overnight at 2-8°C. Secondary Antibody: Donkey Anti-Goat IgG HRP at 1:40,000 at RT for 30 minutes. Block: BlockOut Buffer (p/n MB-073). Exposure: 15 sec. Predicted MW: ~41kDa. Observed MW: ~45kDa.