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Antibodies

Bilirubin Oxidase Antibody Biotin Conjugated

Product Sizes
100 ug
£506.00
200-106-212-0100-100UG
About this Product
SKU:
200-106-212-0100
Additional Names:
BLRO_MYRVE|goat anti-Bilirubin Oxidase biotin conjugation, goat anti-Bilirubin Oxidase Antibody biotin Conjugated
Application:
ELISA, Immunoprecipitation, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
Biotin
Concentration:
1 mg/ml
Extra Details:
Anti-Bilirubin Oxidase antibody is an enzyme that increases the oxidation rate of bilirubin to biliverdin and other substrates. It is ideal for investigators interested in Metabolism or Signal Transduction research.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Goat
Immunogen:
Bilirubin Oxidase [Myrothecium verrucaria]
Isotype:
IgG
Physical State:
Lyophilized
Reactivities:
Yeast/Fungi
Shipping Conditions:
Ambient
Specificity:
Anti-Bilirubin Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purifi
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles., -20[o]C/-70[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Rockland Goat anti Bilirubin Oxidase antibody (200-101-212 lot 6533) was used to detect Bilirubin Oxidase under reducing (R) conditions. A samples of ~1ug of purified protein contained 4% BME and was boiled for 5 minutes and run by SDS-PAGE on a 4-20% acrylamide gel. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 488 conjugated Donkey anti goat (605-741-125 lot 21094 1:10K in TBS/MB-070 1 hr RT) and Dylight 649 conjugated Donkey anti goat (605-743-125 lot 20834 1:10K in TBS/MB-070) 1 hr RT. Images were collected using the BioRad VersaDoc System
Western Blot of Goat anti-Bilirubin Oxidase (Myrothecium verrucaria) Antibody Biotin Conjugated. Lane 1: Bilirubin Oxidase (Myrothecium verrucaria). Load: 50 ng per lane. Primary antibody: Goat anti-Bilirubin Oxidase (Myrothecium verrucaria) Antibody Biotin Conjugated at 1:1,000 overnight at 4°C. Secondary antibody: HRP Streptavidin (p/n S000-03) 1:40,000 in MB-070 for 30 min at RT. Block: MB-070 for 30 minutes at RT. Predicted/Observed size: 64 kDa, 60 kDa for Bilirubin Oxidase. Other band(s): Bilirubin Oxidase splice variants and isoforms.