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Antibodies

Alpha-1-Anti-Trypsin Antibody Biotin Conjugated

Product Sizes
100 ug
£506.00
200-106-147-0100-100UG
About this Product
SKU:
200-106-147-0100
Additional Names:
goat anti-Alpha-1-Trypsin Antibody biotin Conjugation, biotin Conjugated goat anti-Alpha-1-Trypsin Antibody, A1AT antibody, AAT antibody, Alpha 1 antiproteinase antibody, Alpha 1 antitrypsin antibody, Alpha 1 protease inhibitor antibody|SERPINA1
Application:
ELISA, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
Biotin
Concentration:
1 mg/ml
Extra Details:
A member of the serpin family, Anti-Alpha-1-Anti-Trypsin inhibits serine proteases. With a moderate affinity for plasmin and thrombin, Anti-Alpha-1-Anti-Trypsin antibody mainly targets elastase. It inhibits trypsin, chymotrypsin and plasminogen activator by inhibiting insulin-induced NO synthesis. It also decreases coagulation time and has proteolytic activity against insulin and plasmin. The short peptide from AAT also targets elastase, but not trypsin. Anti-Alpha-1-Anti-Trypsin is ideal for researchers involved in Metabolism, Cancer, and Cardiovascular research.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Goat
Immunogen:
a1-Anti-Trypsin [Human Plasma]
Isotype:
IgG
Physical State:
Lyophilized
Reactivities:
Human
Shipping Conditions:
Ambient
Specificity:
Anti-Alpha-1-Anti-Trypsin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially pur
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles., -20[o]C/-70[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of goat anti-Alpha 1 anti trypsin antibody. Lane 1: molecular weight. Lane 2: Human transferrin (green); Alpha 1 anti trypsin (red); and Human IgG (Blue) in a multiplex fluorescent western blot of human serum. Load: 35 ug per lane. Primary antibody: Each primary antibody was diluted to 1:1000 for overnight at 4°C. Secondary antibody: Rabbit IgG (H&L) Antibody DyLight™ 649 Conjugated (green), Goat IgG (H&L) Antibody DyLight™ 549 Conjugated (red), Mouse IgG (H&L) Antibody DyLight™ 488 Conjugated (blue)secondary antibody at 1:10,000 for ~ 1hr at 4 degrees. Block: Blocking Buffer for Fluorescent Western Blotting - MB-070 overnight at 4°C
Western Blot of Anti-Trypsin Polyclonal Antibody-Multiplex Fluorescent Western blot. Lane 1: molecular weight. Lane 2: Alpha-1-Anti-Trypsin (red), Rabbit anti-Transferrin, and Mouse-a-GST were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST. Load: 35 ug per lane. Primary antibody: Each primary antibody at 1:1000 for overnight at 4°C. Secondary antibody: DyLight549 Donkey anti-Rabbit IgG (green), DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) secondary antibody at 1:10,000 for 30 min at RT. Block: MB-070 at 30 min RT.
Western Blot of Goat anti Alpha-1 anti-Trypsin antibody. Lane 1: Alpha-1 anti-Trypsin under reducing conditions. Lane 2: none. Load: 1 ug per lane. Primary antibody: Alpha-1 anti-Trypsin antibody at 1:3000 for overnight at 4°C. Secondary antibody: Dylight 488 conjugated Donkey anti goat secondary antibody at 1:10,000 for 45 min at RT. Block: MB-070 overnight at 4°C. Predicted/Observed size: 55kDa for A Alpha-1 Anti-Trypsin. Other band(s): none.