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Antibodies

Sarcosine Oxidase Antibody Biotin Conjugated

Product Sizes
100 ug
£506.00
200-106-093-0100-100UG
About this Product
SKU:
200-106-093-0100
Additional Names:
goat anti-Sarcosine Oxidase Antibody, biotin Conjugated goat anti-Sarcosine Oxidase Antibody, Sarcosine oxidase subunit alpha, Sarcosine oxidase subunit|soxA
Application:
ELISA, Immunoprecipitation, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
Biotin
Concentration:
1 mg/ml
Extra Details:
Sarcosine Oxidase is an inducible enzyme that catalyzes the oxidative demethylation of sarcosine to glycine, hydrogen peroxide, and 5,10-methylene-tetrahydrofolate in the presence of tetrahydrofolate and oxygen. Sarcosine oxidase is also able to oxidize cyclic amino acids such as L-proline and L-pipecolic acid, albeit at reduced rates.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Goat
Immunogen:
Sarcosine Oxidase [Microbial]
Isotype:
IgG
Physical State:
Lyophilized
Shipping Conditions:
Ambient
Specificity:
Sarcosine Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Sa
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles., -20[o]C/-70[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Rockland Goat anti Sarcosine Oxidase antibody was used to detect Sarcosine Oxidase. Samples of ~1 and 0.25 ug of purified Sarcosine Oxidase per lane were run by SDS-PAGE under reducing and non-reducing conditions and reduced samples contained 4% BME and were boiled for 5 minutes. Protein was transferred to nitrocellulose and probed with Rockland Goat anti Sarcosine Oxidase (200-101-093 lot 5951 1:5K in MB-0070, ON 4 C). Primary antibody was detected with Rockland Dylight 649 conjugated Donkey anti Goat (605-743-125 1:10K 1.5 hr RT in MB-070 and imaged on the BioRad VersaDoc imaging system.
Western Blot of Goat anti-Sarcosine Oxidase (Microbial) Antibody Biotin Conjugated. Lane 1: Sarcosine Oxidase (Microbial). Load: 50 ng per lane. Primary antibody: Goat anti-Sarcosine Oxidase (Microbial) Antibody Biotin Conjugated at 1:1,000 overnight at 4°C. Secondary antibody: HRP streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 45 kDa, 45 kDa for Sarcosine Oxidase monomeric alpha subunit.