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Antibodies

Alpha-1-Anti-Trypsin Antibody Peroxidase Conjugated

Product Sizes
100 ug
200-103-147-0100-100UG
About this Product
SKU:
200-103-147-0100
Additional Names:
goat anti-Alpha-1-Trypsin Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Alpha-1-Trypsin Antibody, A1AT antibody, AAT antibody, Alpha 1 antiproteinase antibody, Alpha 1 antitrypsin antibody, Alpha 1 protease inhibitor antibody|SERPINA1
Application:
ELISA, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
HRP
Concentration:
1 mg/ml
Extra Details:
Anti-Alpha 1-Anti-Trypsin Antibody detects a1-Anti-Trypsin protein. a1-Anti-Trypsin is an inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Anti-Trypsin irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form of Antitrypsin inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Defects in SERPINA1 are the cause emphysema, liver cirrhosis and liver failure. Environmental factors, particularly cigarette smoking, greatly increase the risk of emphysema at an earlier age. Anti-Alpha-1-Anti-Trypsin Antibody is ideal for investigators involved in Cell Signaling, Signal Transduction and Cancer research.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Goat
Immunogen:
Anti-alpha 1 Anti-Trypsin Antibody was produced by repeated immunizations with native A Alpha1-Anti-Trypsin protein from human plasma.
Isotype:
IgG
Physical State:
Lyophilized
Reactivities:
Human
Shipping Conditions:
Ambient
Specificity:
Anti-alpha-1-Anti-Trypsin Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of Goat-anti-Alpha-1-Anti-Trypsin Antibody. Lane 1: molecular weight. Lane 2: Goat-anti-Alpha-1-Anti-Trypsin (red), Rabbit anti-Transferrin, and Mouse-a-GST. Load: 35 ug per lane under reducing (R) conditions (+4% BME) in albumin depleted human serum with 320 ng of added GST. Primary antibody: Alpha 1 Anti-Trypsin antibody at 1:400 for overnight at 4°C. Secondary antibody: DyLight 649 Donkey anti-Goat IgG (red) secondary antibody at 1:10,000 for 30 min at RT in MB-070 30 buffer. Block: 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4°C. Predicted/Observed size: 46.7kDa, ~55kDa for Alpha-1-AntiTrypsin. Other band(s): DyLight 488 Donkey anti-Mouse IgG (blue) and DyLight549 Donkey anti-Rabbit IgG (green) secondary antibodies.
Western Blot of Goat Anti-ALPHA 1 ANTI TRYPSIN Antibody Lane 1: reduced Alpha-1anti-Trypsin. Lane 2: none. Load: ~1ug per lane. Primary antibody: Alpha-1-Anti-Trypsin antibody at 1:3000 for overnight at 4°C. Secondary antibody: Dylight 488 conjugated Donkey anti goat secondary antibody at 1:10,000 for 1.5 hr at RT. Block: MB-070 buffer overnight at 4°C. Predicted/Observed size: 46.7kDa, ~55kDa for Alpha-1-AntiTrypsin. Other band(s): none.
ELISA Results of Goat Anti-Alpha-1-Anti-Trypsin Peroxidase Conjugated Antibody. Each well was coated in duplicate with 1.0 ug of Alpha-1-Anti-Trypsin. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugated Stabilizer (p/n MB-076), Goat Anti-Alpha-1-Anti-Trypsin Peroxidase Conjugated Antibody (p/n 200-103-147) and TMB substrate (p/n TMBE-1000).