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Antibodies

Peroxidase Antibody

Product Sizes
100 ug
200-101-038-100UG
About this Product
SKU:
200-101-038
Additional Names:
goat anti-Peroxidase Antibody, goat anti-HRP antibody, horseradish antibody, goat anti Peroxidase, goat anti HRP|PRXC, HRPC
Application:
ELISA, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Peroxidase described as the removal of hydrogen peroxide, oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, a response to environmental stresses such as wounding, a and pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Goat
Immunogen:
Peroxidase (Horseradish)
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Label/Epitope-tag
Shipping Conditions:
Dry Ice
Specificity:
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Peroxidase (Horserad
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Rockland Goat anti Peroxidase antibody was used to detect horse radish peroxidase under reducing and non-reducing conditions. Reduced samples of purified horseradish peroxidase contained 4% BME and were boiled for 5 minutes. Samples of ~1 and 0.25 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with Rockland Goat anti Peroxidase antibody (p/n 200-1138, lot 25614) at 1:5K in MB-070, ON 4°C. Primary antibody was detected with Rockland Dylight 649 conjugated Donkey anti-Goat (p/n 605-743-125, lot 20834) at 1:10K 1.5 hr RT in MB-070 and imaged on the BioRad VersaDoc imaging system.
Western Blot of Goat anti-Peroxidase Antibody. Lane 1: Peroxidase. Lane 2: None. Load: 50 ng per lane. Primary antibody: Peroxidase primary antibody at 1:1,000 overnight at 4°C. Secondary antibody: DyLight™ 649 goat secondary antibody (p/n 605-743-125) at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 44 kDa, 44 kDa for Peroxidase. Other band(s): None.