Fluorescent TrueBlot[R]: Anti-Rabbit IgG DyLight[TM] 800
Product Sizes
100 ul
18-4516-32-100UL
About this Product
- SKU:
- 18-4516-32
- Additional Names:
- Anti-Rabbit IgG DL800, TrueBlot, DL800 TrueBlot ULTRA, DyLight™ 800 TrueBlot, TrueBlot for IP/WB, TrueBlot for immunoprecipitation, TrueBlot for western blotting, Fluorescent TrueBlot, Rb TrueBlot, Infrared, IR, NIR, IR800
- Application:
- Dot Blot, FLISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
- Buffer:
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
- CE/IVD:
- RUO
- translate.label.attr.clone:
- eB182
- Clonality:
- Monoclonal
- Conjugate:
- DyLight™ 800
- Concentration:
- 1 mg/ml
- Extra Details:
- Rabbit IgG TrueBlot® is a unique DyLight™ 800 conjugated Anti-rabbit IgG monoclonal secondary antibody. Rabbit IgG TrueBlot® enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate publication-quality IP/Fluorescent Western Blot data with Rabbit IgG TrueBlot®, simply substitute the conventional DL800 Anti-rabbit IgG blotting reagent with Fluorescent Rabbit TrueBlot® Antibody DyLight™ 800 and follow the prescribed protocol for sample preparation and immunoblotting. Ideal for Li Cor Odyssey imaging as well as other IR and near IR imaging systems. Rabbit IgG TrueBlot® is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot preferentially detects the non-reduced form of rabbit IgG over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Rabbit IgG TrueBlot® with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/Western blot applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions.
- Formulation:
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- Host:
- Mouse
- Isotype:
- IgG
- Physical State:
- Lyophilized
- Reactivities:
- Rabbit
- Shipping Conditions:
- Ambient
- Specificity:
- Fluorescent Rabbit TrueBlot® Antibody DyLight™ 800 Conjugate was prepared from tissue culture supernatant by Protein G affinity chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against Anti-Rabbit Serum. Reactivity is observed against native Rabbit IgG by both Western blot and ELISA.
- Storage Conditions:
- Please refer to datasheet
- Supplier:
- Rockland Inc
- Type:
- Antibody: Secondary Antibodies
- Manufacturer's Data Sheet:https://www.rockland.com/search/?searchString=18-4516-32
