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Antibodies

Mouse IgG Fc Antibody

Product Sizes
2 ml
110-1103-2ML
About this Product
SKU:
110-1103
Additional Names:
goat anti-Mouse IgG F(c) fragment Antibody, goat anti-Mouse IgG Fc fragment Antibody, rabbit anti Mouse IgG Fc
Application:
ELISA, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
85 mg/ml
Extra Details:
Anti-Mouse IgG F(c) generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH. Receptors bind the Fc portion of mouse IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.
Format:
Antiserum
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Goat
Immunogen:
Mouse IgG F(c) fragment
Physical State:
Lyophilized
Reactivities:
Mouse
Shipping Conditions:
Ambient
Specificity:
IgG Fc|This product was prepared from monospecific antiserum by delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum. No reaction was observed against Mouse IgG F(ab).
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
KIF20B knockdown inhibits cell proliferation. (D) Expression of proliferation marker PCNA in shRNA cells and control cells, measured by western Blotting. Primary antibodies used mouse anti-(PCNA) proliferating cell nuclear antigen [1:1000] and mouse anti-B Beta-actin [1:500] with goat anti-mouse Fc secondary antibody [1:10,000] p/n 110-1103. Knocking down KIF20B inhibited PCNA expression. *P<0.05 vs. control. KIF, kinesin family member; PCNA, proliferating cell nuclear antigen; shRNA, short hairpin RNA. Figure 3. PMID: 30664160.
KIF20B shRNA efficiency. (B) The shRNA efficiency measured by western blotting. Primary antibodies used rabbit anti-KIF20B antibody [1:100] and mouse anti-B Beta-actin [1:500] with goat anti-rabbit [1:10,000] p/n 711-1122 and goat anti-mouse Fc secondary antibody [1:10,000] p/n 110-1103. *P<0.05 vs. control. KIF, kinesin family member; shRNA, short hairpin RNA. Figure 2. PMID: 30664160.