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Antibodies

Superoxide Dismutase Antibody

Product Sizes
2 ml
100-4191-2ML
About this Product
SKU:
100-4191
Additional Names:
rabbit anti-Superoxide Dismutase Antibody, ALS 1 antibody, ALS antibody, ALS1 antibody, Amyotrophic lateral sclerosis 1 adult antibody, Amyotrophic lateral sclerosis 1 antibody, Cu/Zn SOD antibody, Cu/Zn superoxide dismutase antibody, Homodimer antibody|SOD1
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
70 mg/ml
Extra Details:
Superoxide Dismutase destroys radicals which are normally produced within the cells and which are toxic to biological systems.
Format:
Antiserum
Formulation:
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Superoxide Dismutase [Bovine Erythrocytes]
Physical State:
Lyophilized
Reactivities:
Bovine
Shipping Conditions:
Ambient
Specificity:
This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Superoxide Dismutase [Bovine Erythrocytes]. Cross reactivity against Superoxide Dismutase from other tissues and species may occur but have not been specifically determined.
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles., -20[o]C/-70[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Wester Blot using Anti-Superoxide Dismutase (RABBIT) Antibody. Dose-response RT-PCR analysis of m-RNA levels for antioxidant enzymes. (a) The up-regulation of SOD and CAT m-RNAs in comparison to untreated control after a 3-h stimulation by peptides CMX-8933 and CMX-9236 as a function of peptide dose (ng/ml) in primary neuron-glia rat cortical cultures. The gel electrophoresis data show increases in m-RNAs for SOD (208 bp amplicon) and CAT (95 bp amplicon) m-RNAs, whereas GAPDH m-RNA (451 bp amplicon), the constituitively expressed housekeeping control, remained constant. (b) The quantitation of the data from three independent experiments (histobars represent the means, error bars denote standard deviations) by laser densitometry scans; all values are relative to untreated controls (n=3). bMQ denotes the 100-bp marker. Arrows on the left denote the sizes of marker bands in bp. Fig 1. PMID: 15451365.
Western Blot of Anti-Superoxide Dismutase (RABBIT) Antibody. Western blot studies of SOD enzyme protein up-regulation in vitro. Primary rat cortical cultures were incubated with 1, 10, and 100 ng/ ml peptides for 5 h prior to analysis by gel electrophoresis and Western blots. Upper panels show the SDS gels; lower panels show the averages of three independent experiments (n=3) analyzed by laser densitometry scans (error bars denote standard deviations). Arrows on the left denote the sizes (kDa) of marker proteins. Up-regulation of all three enzyme proteins occurs following treatments with CMX-8933 and CMX-9236 peptides. Fig 3. PMID: 15451365.
Western Blot of Anti-Superoxide Dismutase (RABBIT) Antibody. Western blot assays of up-regulation of SOD proteins in vivo. Sprague-Dawley rats were injected with 1 or 6 mg/kg of CMX-9236, and total cellular protein extracts were prepared from brain and heart tissue at 5 h post tail vein IV injections of peptide. Upper panels represent typical immunoblots for each protein assayed. The arrows on the left denote the sizes (kDa) of marker proteins. Lower panels show the quantitation of three independent experiments (histobars denote means, and error bars the standard deviations). The Western blot data show increases at 31kDa corresponding to the monomeric SOD-1 protein. Fig 4. PMID: 15451365.