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Antibodies

AML1-ETO Antibody

Product Sizes
100 ul
£638.00
100-401-V33-100UL
About this Product
SKU:
100-401-V33
Additional Names:
Runt-related transcription factor 1, Acute myeloid leukemia 1 protein, Core-binding factor subunit alpha-2, CBF-alpha-2, Oncogene AML-1, Polyomavirus enhancer-binding protein 2 alpha B subunit, PEA2-alpha B, PEBP2-alpha B, SL3-3 enhancer factor 1 alpha B subunit, SL3/AKV core-binding factor alpha B subunit|RUNX1
Application:
ChIP, ELISA, Western Blot
Buffer:
None
CE/IVD:
RUO
Clonality:
Polyclonal
Extra Details:
This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22. This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukemia. It produces a chimerical gene made up of the 5'-region of AML1and the 3'-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Anti-AML1-ETO Antibody is ideal for research in Cancer and Gene Expression.
Format:
Antiserum
Host:
Rabbit
Immunogen:
Anti-AML1-ETO Antibody was produced in rabbits by repeated immunizations with a synthetic peptide of human AML1-ETO.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Dry Ice
Specificity:
Anti-AML1-ETO Antibody is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human AML1-ETO. Cross reactivity with AML1-ETO from other sources is not known.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles., 2-8[o]C diluted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Chromatin Immunoprecipitation results of Rabbit Anti-AML1-ETO Antibody. Chromatin from 1.25 million formaldehyde cross-linked Kasumi-1 cells was used with 4ul of Anti-AML1-ETO Antibody and 20ul of magnetic IgG beads per immunoprecipitation. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. ChIP results shows the occupancy, calculated as the ratio + control/background for which the H2B gene was used.
ELISA results of Rabbit anti-AML1-ETO antibody. Antigen: BSA conjugated AML1-ETO. Coating amount: 0.1 ug per well. Dilution series: serial dilution. Estimated Antibody Titer to be 1:32,750. Substrate: TMB (p/n TMBE-1000).
Western blot results of Rabbit Anti-AML1-ETO antibody. Lane 1: SKNO-1 Nuclear extract lysates. Load: 15 ug. Primary antibody: AML1-ETO antibody at 1:1000 overnight at 4°C. Secondary antibody: Goat Anti-rabbit HRP secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO/TBST overnight at 4°C.
ChIP-seq results obtained with the antibody directed against AML1-ETO. ChIP was performed as described in figure 1. The IP'd DNA from 6 ChIP's were pooled and analyzed with an Illumina Genome Analyzer. Library preparation, cluster generation, and sequencing were performed according to the manufacturer's instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3. Figure 3-5 shows three genomic regions region surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.
ChIP-seq results obtained with the antibody directed against AML1-ETO. ChIP was performed as described in figure 1. The IP'd DNA from 6 ChIP's were pooled and analyzed with an Illumina Genome Analyzer. Library preparation, cluster generation, and sequencing were performed according to the manufacturer's instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3. Figure 3-5 shows three genomic regions region surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.