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Antibodies

Cyclin D1 Antibody

Product Sizes
100 ul
100-401-153-100UL
About this Product
SKU:
100-401-153
Additional Names:
CCND1|rabbit anti-cyclin D1 antibody, G1/S-specific cyclin-D1, PRAD1 oncogene, BCL-1 oncogene
Application:
ELISA, Immunoprecipitation, Western Blot
Buffer:
None
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
85 mg/ml
Extra Details:
Cyclin D1 (also known as G1/S-specific cyclin D1, PRAD1 oncogene, BCL-1 oncogene, and PRAD1: parathyroid adenomatosis 1) is encoded by a gene that belongs to the highly conserved cyclin family. Cyclins are characterized by a dramatic periodicity in protein abundance throughout the cell cycle and function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns, which contribute to the temporal coordination of each mitotic event. Cyclin D1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.
Format:
Antiserum
Host:
Rabbit
Immunogen:
Anti-Cyclin D1 was produced by repeated immunizations of full length fusion protein corresponding to the human gene sequence.
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Dry Ice
Specificity:
This product was prepared from monospecific antiserum by delipidation and defibrination. Antiserum will specifically react with a 40-45 kDa Cyclin D1 protein from human, rat and mouse tissue. No reaction was observed against other related cyclins. Cross reactivity with Cyclin D1 from other species may also occur.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
A model summarizes the interactions between cyclins and the cyclin dependent kinases in multicellular animal cells. The R point is the restriction point. The diagram shows the stages of the cell cycle and the binding of the specified cyclins with the corresponding CDKs at each stage. cdc2 is kinase, p107 and E2F are proteins involved in transcription. See Pines, J. (1993).
Western blot analysis is shown using Rockland's Anti-Cyclin D1 antibody to detect Human Cyclin D1 present in asynchronous HN30 cell lysates. HN30 cells, are from head and neck cancer cells that over express cyclin B1 and D1. Comparison to a molecular weight marker indicates a band of ~34 kDa corresponding to the expected molecular weight for the protein (arrowhead). The blot was incubated with a 1:500 dilution of the antibody at room temperature. Detection occurred using a 1:10,000 of HRP conjugated Goat-a-Rabbit IgG (p/n 611-103-122) and chemiluminescence reagent with a 1-min exposure time. Other detection systems will yield similar results. Personal communication Luca Cote.