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Antibodies

Beta-Phosphoglucomutase Antibody

Product Sizes
2 ml
100-101-237-2ML
About this Product
SKU:
100-101-237
Additional Names:
goat anti-Beta-Phosphoglucomutase Antibody, Beta PGM antibody, Glucose phosphomutase 1 antibody, Glucose phosphomutase antibody, PGM antibody, pgmB antibody|pgmB
Application:
ELISA, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
90 mg/ml
Extra Details:
Beta-Phosphoglucomutase catalyzes the interconversion of D-glucose 1-phosphate (G1P) and D-glucose 6-phosphate (G6P), forming beta-D-glucose 1,6-(bis)phosphate (beta-G16P) as an intermediate. The beta-phosphoglucomutase (Beta-PGM) acts on the beta-C1 anomer of G1P. Glucose or lactose are used in preference to maltose, which is only utilized after glucose or lactose has been exhausted. It plays a key role in the regulation of the flow of carbohydrate intermediates in glycolysis and the formation of the sugar nucleotide UDP-glucose. The catalysis proceeds via a phosphoenzyme formed by reaction of an active-site nucleophile with the cofactor glucose 1,6-diphosphate (G1,6-diP). The phosphorylated mutase binds either G1P or G6P and transfers the phosphoryl group to the C6OH or C1OH, respectively.
Format:
Antiserum
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Goat
Immunogen:
Beta Phosphoglucomutase [Lactococcus lacti]
Physical State:
Lyophilized
Reactivities:
Bacteria
Shipping Conditions:
Ambient
Specificity:
This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified b-Phosphoglucomutase [Lactococcus lacti]. Cross reactivity against b -Phosphoglucomutase from other sources is unknown.
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Rockland Goat anti antibody (200-101-237 lot 8180) was used to detect purified Beta Phospho Glucomutase under reducing (R) and non-reducing (NR) conditions. Reduced samples of protein contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 488 conjugated Donkey anti goat (605-741-125 lot 21094 1:10K in TBS/MB-070 1 hr RT). Images were collected using the BioRad VersaDoc System.