Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

Armenian Hamster IgG isotype Control

Product Sizes
1 mg
£160.00
019-001-002-1MG
About this Product
SKU:
019-001-002
Additional Names:
Armenian Hamster IgG isotype Control, A. Hamster IgG control, Control Protein, Isotype Protein
Application:
FLISA, Flow Cytometry, Immunofluorescence, SDS-PAGE
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Concentration:
1 mg/ml
Extra Details:
Armenian Hamster isotype controls are used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In hamster there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain alpha - IgA, gamma - IgG 1, 2a, 2b, 3 and u - IgM, light chain kappa and lambda.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Physical State:
Lyophilized
Shipping Conditions:
Ambient
Specificity:
ARMENIAN HAMSTER IgG whole molecule was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Armenian Hamster IgG. Greatly diminished reactivity will occur against anti-Golden Sy
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles., -20[o]C/-70[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Isotype Controls
Anti-CD154-facilitated alloengraftment is multilineage. Twenty mice from 2 representative experiments were phenotyped at 120 days after BMT for donor-host origin of CD4+ and CD8+ T cells, CD19+ B cells, and MAC-1+ myeloid cells. On the x-axis are shown the host and donor proportions of each of the lineages. indicates the proportion of each lineage of host origin; , the proportion of each lineage that is of donor origin. On the y-axis is shown the percentage of PBLs of each lineage. Irrelevant hIgG-treated mice had no detectable donor chimerism and thus are composed entirely of host-type cells. Note that most CD4+ T cells, CD19+ B cells, and MAC-1+ myeloid cells in anti-CD154-treated mice are of donor origin. In contrast, most of the CD8+ T cells are of host origin. irrelevant hamster IgG (hIgG) (p/n 019-001-002). Fig. 3. PMID: 11435318.
SDS PAGE Results of Armenian Hamster IgG Whole Molecule Isotype Control. Lane 1: Armenian Hamster IgG Reduced [5.0ug]. Lane 2: Armenian Hamster IgG Reduced [1.0ug]. Lane 3: Lane 4: Armenian Hamster IgG Non-Reduced [1.0ug]. Lane 5: Armenian Hamster IgG Non-Reduced [5.0ug]. 4-20% Gel, Coomassie Stained.