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Antibodies

Monkey IgM

Product Sizes
250 ug
£310.00
017-0107-250UG
About this Product
SKU:
017-0107
Additional Names:
Monkey Immunoglobulin M
Application:
ELISA, Immunohistochemistry, SDS-PAGE, Western Blot
Buffer:
0.1 M Tris Chloride, 0.5 M Sodium Chloride
CE/IVD:
RUO
Concentration:
1 mg/ml
Extra Details:
Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum.
Formulation:
0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
Isotype:
IgM
Physical State:
Liquid
Shipping Conditions:
Blue Ice
Specificity:
Monkey IgM whole molecule was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Monkey IgM whole molecule was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Monkey Serum and anti-Monkey IgM (u chain specif
Storage Conditions:
-20[o]C 50% glycerol. Aliquot. Avoid freeze/thaw cycles., 2-8[o]C diluted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Isotype Controls
Humoral immune response. Plasmas were analyzed before vaccination (week 0, pre-immune) and at weeks 1, 2, 4 and 6 post-vaccination for anti-VSV antibodies. Neutralizing antibody (NA) titer (left panel) was defined as the dilution of serum that inhibited infection of mouse EL4 cells with M51R-eGFP by 50%. IgM [p/n 017-0107] (middle panel) and IgG [p/n 017-0102] (right panel) levels are expressed in ng/mL (mean +/- SD, triplicate values), measured by ELISA with extrapolation from standard curves. Data for low dose (a) and high dose (b) vaccinations with M51R and M51R-F are shown. Note scale differences for low and high dose data. Statistical significance was determined by two-factor analysis of variance with cohort and time as the two factors. For all three antibody types, statistical significance of p < 0.05 (after correction for multiple comparisons) was obtained for comparisons of cohort 1 versus cohort 3 (low dose M51R vs. M51R-F), cohort 1 versus cohort 2 (low dose M51R vs. high dose M51R), and cohort 3 versus cohort 4 (low dose M51R-F vs. high dose M51R-F), but not for cohort 2 versus cohort 4 (high dose M51R vs. high dose M51R-F). The data shown represent 1 of 2 analyses with similar results performed on plasma from each animal. Figure 1. PMID: 29562688.
SDS-PAGE of Monkey IgM Whole Molecule. Lane 1: Monkey IgM, Non-Reduced. Lane 2: Monkey IgM, Reduced. Load: 1.0 ug per lane. Predicted/Observed size - Non-Reduced: 900 kDa (Pentamer), 900 kDa (Molecule larger than can pass through gel), Reduced: 78 and 25 kDa, 75 and 25 kDa.