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Antibodies

Monkey IgG

Product Sizes
2 mg
£ POA
017-0102-2MG
About this Product
SKU:
017-0102
Additional Names:
Monkey immunoglobulin G
Application:
ELISA, Immunohistochemistry, SDS-PAGE, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Concentration:
2.2 mg/ml
Extra Details:
Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-afinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype:
IgG
Physical State:
Lyophilized
Shipping Conditions:
Ambient
Specificity:
Monkey IgG whole molecule was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Monkey IgG whole molecule was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Monkey IgG and anti-Monkey Serum.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles., 2-8[o]C diluted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Isotype Controls
Humoral immune response. Plasmas were analyzed before vaccination (week 0, pre-immune) and at weeks 1, 2, 4 and 6 post-vaccination for anti-VSV antibodies. Neutralizing antibody (NA) titer (left panel) was defined as the dilution of serum that inhibited infection of mouse EL4 cells with M51R-eGFP by 50%. IgM [p/n 017-0107] (middle panel) and IgG [p/n 017-0102] (right panel) levels are expressed in ng/mL (mean +/- SD, triplicate values), measured by ELISA with extrapolation from standard curves. Data for low dose (a) and high dose (b) vaccinations with M51R and M51R-F are shown. Note scale differences for low and high dose data. Statistical significance was determined by two-factor analysis of variance with cohort and time as the two factors. For all three antibody types, statistical significance of p < 0.05 (after correction for multiple comparisons) was obtained for comparisons of cohort 1 versus cohort 3 (low dose M51R vs. M51R-F), cohort 1 versus cohort 2 (low dose M51R vs. high dose M51R), and cohort 3 versus cohort 4 (low dose M51R-F vs. high dose M51R-F), but not for cohort 2 versus cohort 4 (high dose M51R vs. high dose M51R-F). The data shown represent 1 of 2 analyses with similar results performed on plasma from each animal. Figure 1. PMID: 29562688.
SDS PAGE of Monkey IgG Whole Molecule. Lane 1: Non-Reduced Monkey IgG Whole Molecule. Lane 2: 5 uL Opal Prestained Marker (MB-210-0500). Lane 3: Reduced Monkey IgG Whole Molecule. Load: 1 ug per lane. Predicted/Observed size: Non-Reduced at 160 kDa, Observed at greater than 180; Reduced at 55, 25 kDa. Non-reduced sample migrates higher due to large molecular weight.
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