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Antibodies

Rat IgM

Product Sizes
1 mg
£266.00
012-0107-1MG
About this Product
SKU:
012-0107
Additional Names:
Rat Immunoglobulin M
Application:
ELISA, Immunohistochemistry, SDS-PAGE, Western Blot
Buffer:
0.1 M Tris Chloride, 0.5 M Sodium Chloride
CE/IVD:
RUO
Concentration:
1.1 mg/ml
Extra Details:
Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum.
Formulation:
0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
Isotype:
IgM
Physical State:
Liquid
Shipping Conditions:
Blue Ice
Specificity:
Rat IgM whole molecule was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Rat IgM whole molecule assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat Serum and anti-Rat IgM (u chain specific). No reaction
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles., 2-8[o]C diluted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Isotype Controls
SDS-Page of Rat IgM Whole Molecule. Lane 1: Rat IgM, Non-Reduced. Lane 2: Rat IgM, Reduced. Load: 1.0 ug per lane. Predicted/Observed size-Predicted/Observed size - Non-Reduced: 900 kDa (Pentamer), 900 kDa (Molecule larger than can pass through gel), Reduced: 78 and 25 kDa, 78 and 25 kDa.
SDS-PAGE of Rat IgG F(c) Fragment Rhodamine Conjugated (p/n 012-0003). Lane M: 3 uL Opal Prestained Marker (p/n MB-210-0500). Lane 1: Reduced Rat IgG Whole Molecule (p/n 012-0102). Lane 2: Reduced Rat IgG F(c) Fragment Rhodamine Conjugated (p/n 012-0003). Lane 3: Reduced Rat IgG Fab Fragment (p/n 012-0105). Lane 4: Reduced Rat IgM Whole Molecule (p/n 012-0107). Load: 1 ug of IgG, F(c), Fab; 1.5 ug of IgM. Predicted/Observed size: IgG at 55 and 25 kDa; F(c) at 25 kDa; Fab at 25 kDa; IgM at 78 and 25 kDa. Observed F(c) Fragment migrates slightly higher.