Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

Rat IgG Fc

Product Sizes
1 mg
£259.00
012-0103-1MG
About this Product
SKU:
012-0103
Additional Names:
Rat Immunoglobulin G F(c) fragment, IgG Fc
Application:
ELISA, Flow Cytometry, Immunohistochemistry, SDS-PAGE, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Concentration:
1 mg/ml
Extra Details:
Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The F(c) fragment binds with very high affinity to the Fc receptor proteins on phagocytic leukocytes. When digested from the whole antibody molecule, the F(c) fragment no longer posses the epitope recognition site.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype:
IgG
Physical State:
Liquid
Shipping Conditions:
Blue Ice
Specificity:
Rat IgG F(c) fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Rat IgG F(c) fragment was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat Serum, anti-Rat Ig
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Antibody: Isotype Controls
B cell development was reconstituted in the Crescendo Mouse. (a) Flow cytometric analysis of bone marrow cells stained with anti-B220 antibody (horizontal axis) and antibodies to differentiation markers (vertical axis), CD117, CD43, Ly51, CD25, IgM-Fc (TKO & wt) or IgG-Fc (Crescendo Mouse); (b) photograph of spleens from wild type mouse, TKO mouse and Crescendo Mouse; (c) sandwich ELISA detecting serum Ig FcG Gamma chains in TKO (circles), wild type (squares) and the Crescendo Mouse (triangles). Rat Fc fragments (p/n 012-0103). Fig. 2. PMID: 31600579.
SDS-Page of Rat IgG F(c). Lane 1: Rat Fc - Non-reduced. Lane 2: Rat Fc - Reduced. Load: 1.0 ug per lane. Predicted/Observed size: 25 kDa, 25 kDa for Reduced Fc. Other band(s): None.
SDS-PAGE of Rat IgG F(c) Fragment Rhodamine Conjugated (p/n 012-0003). Lane M: 3 uL Opal Prestained Marker (p/n MB-210-0500). Lane 1: Reduced Rat IgG Whole Molecule (p/n 012-0102). Lane 2: Reduced Rat IgG F(c) Fragment Rhodamine Conjugated (p/n 012-0003). Lane 3: Reduced Rat IgG Fab Fragment (p/n 012-0105). Lane 4: Reduced Rat IgM Whole Molecule (p/n 012-0107). Load: 1 ug of IgG, F(c), Fab; 1.5 ug of IgM. Predicted/Observed size: IgG at 55 and 25 kDa; F(c) at 25 kDa; Fab at 25 kDa; IgM at 78 and 25 kDa. Observed F(c) Fragment migrates slightly higher.