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Antibodies

Rat IgG whole molecule

Product Sizes
1 g
£ POA
012-0102-1000-1G
About this Product
SKU:
012-0102-1000
Additional Names:
Rat Immunoglobulin G, Rat IgG whole molecule, Rat IgG purified protein
Application:
ELISA, Flow Cytometry, SDS-PAGE, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Concentration:
40 mg/ml
Extra Details:
Rat Immunoglobulin purified protein are antibody molecules. Rat IgG is composed of four peptide chains - two heavy chains (gamma) and two light chains. Rat IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. Rat IgG typically constitutes 75% of serum immunoglobulins. Rat IgG molecules are synthesized and secreted by plasma B cells.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype:
IgG
Physical State:
Liquid
Shipping Conditions:
Dry Ice
Specificity:
Rat IgG was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG and anti-Rat Serum.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Antibody: Isotype Controls
SDS PAGE of Rat IgG Whole Molecule. Lane 1: Non-Reduced Rat IgG Whole Molecule. Lane 2: 5 uL Opal Prestained Marker (p/n MB-210-0500). Lane 3: Reduced Rat IgG Whole Molecule. Load: 1 ug per lane. Predicted/Observed size: Non-Reduced at 160kDa/Observed at 245 kDa; Reduced at 55, 25 kDa. Non-reduced IgG migrates slightly higher.
SDS-PAGE of Rat IgG F(c) Fragment Rhodamine Conjugated (p/n 012-0003). Lane M: 3 uL Opal Prestained Marker (p/n MB-210-0500). Lane 1: Reduced Rat IgG Whole Molecule (p/n 012-0102). Lane 2: Reduced Rat IgG F(c) Fragment Rhodamine Conjugated (p/n 012-0003). Lane 3: Reduced Rat IgG Fab Fragment (p/n 012-0105). Lane 4: Reduced Rat IgM Whole Molecule (p/n 012-0107). Load: 1 ug of IgG, F(c), Fab; 1.5 ug of IgM. Predicted/Observed size: IgG at 55 and 25 kDa; F(c) at 25 kDa; Fab at 25 kDa; IgM at 78 and 25 kDa. Observed F(c) Fragment migrates slightly higher.
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