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Antibodies

Rat IgG1 isotype control Biotin

Product Sizes
50 ug
£147.00
012-006-040-50UG
About this Product
SKU:
012-006-040
Additional Names:
Rat Isotype Control, Rat IgG1 Biotin isotype, RAT IgG1 subclass isotype control
Application:
Dot Blot, ELISA, Flow Cytometry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
translate.label.attr.clone:
RG1
Conjugate:
Biotin
Concentration:
1 mg/ml
Extra Details:
RAT IgG1 isotype control is used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In Rat there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain alpha - IgA, gamma - IgG 1, 2a, 2b, 2c and u - IgM, light chain kappa and lambda.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype:
IgG1
Physical State:
Lyophilized
Shipping Conditions:
Ambient
Specificity:
RAT IgG1 isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein G. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rat IgG, and anti-Rat serum. Isotyping assay resulted non-reactive with antisera to Rat IgG2a, IgG2b, IgG3, IgM, and IgA. Biotin Conjugated Rat IgG1 confirmed by Dot Blot.
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles., -20[o]C/-70[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Isotype Controls