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Proteins and Peptides

rRat SCF Protein

Product Sizes
100 ug
£680.00
012-001-W20-0100-100UG
About this Product
SKU:
012-001-W20-0100
Additional Names:
c-Kit Ligand, Hematopoietic growth factor KL, KL, Steel Factor, Stem cell factor (SCF), Mast cell growth factor(MGF)|Kitlg
Application:
Cell-based/Functional Assay, SDS-PAGE
Buffer:
0.1% Trifluoroacetic acid
CE/IVD:
RUO
Extra Details:
Stem Cell Factor (SCF) is a cytokine made by fibroblasts and endothelial cells. SCF binds to the receptor known as c-Kit (CD117) and is thought to play a critical role in the maintenance or survival of hematopoietic stem cells. Human SCF shows no activity on murine cells, but murine and rat SCF are active on human cells. Recombinant rat SCF is a non-glycosylated protein, containing 165 amino acids, with a molecular weight of 18.4 kDa.
Formulation:
0.1% Trifluoroacetic acid
Immunogen:
Kitlg
Physical State:
Lyophilized
Purity:
>98%
Shipping Conditions:
Ambient
Specificity:
Stem Cell Factor purity was determined to be greater than 98% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
SDS-PAGE of Rat Stem Cell Factor Recombinant Protein. Lane 1: 1 ug Rat SCF in non-reducing conditions (-). Lane 2: 1 ug Rat SCF in reducing conditions (+). Lane 3: Molecular weight marker. Rat SCF has a predicted MW of 18.4 kDa.
Bioactivity of Rat Stem Cell Factor Recombinant Protein. Serial dilutions of Rat SCF, starting at 250 ng/mL, were added to TF-1 cells growing in GM-SCF free media. Cell proliferation was measure after 63 hours and the linear portion of the curve was us used to calculate the ED50. The ED50 of Rat SCF is between 10-15 ng/mL. This value is comparable to the typical expected range of 20-40 ng/mL.