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Proteins and Peptides

rRat CCL2 Protein

Product Sizes
10 ug
£319.00
012-001-V74-0010-10UG
About this Product
SKU:
012-001-V74-0010
Additional Names:
Ccl2|Immediate-early serum-responsive protein JE, Monocyte chemoattractant protein 1, MCAF, Monocyte chemotactic protein 1 (MCP-1), Small-inducible cytokine A2
Application:
Cell-based/Functional Assay, SDS-PAGE
Buffer:
0.1% Trifluoroacetic acid
CE/IVD:
RUO
Extra Details:
Monocyte Chemotactic Protein 1 (MCP-1), also known as CCL2, is thought to be produced by injured or infected tissues. MCP-1 signals through G protein-coupled receptors, CCR2 and CCR4, to recruit memory T cells, monocytes and dendritic cells. Recombinant rat MCP-1is a non-glycosylated protein, containing 125 amino acids, with a molecular weight of 14.1 kDa.
Formulation:
0.1% Trifluoroacetic acid
Immunogen:
Ccl2
Physical State:
Lyophilized
Purity:
>98%
Shipping Conditions:
Ambient
Specificity:
Monocyte Chemotactic protein-1 (CCL2) purity was determined to be greater than 98% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
SDS-PAGE of Rat Monocyte Chemotactic Protein-1 (CCL2) Recombinant Protein. Lane 1: Molecular weight marker. Lane 2: 1 ug Rat MCP-1 in non-reducing conditions (-). Lane 3: 1 ug Rat MCP-1 in reducing conditions (+). Rat MCP-1 has a predicted MW of 14.1 kDa.
Bioactivity of Rat Monocyte Chemotactic Protein-1 (CCL2) Recombinant Protein. Human THP-1 cells were allowed to migrate to Rat MCP-1 at (0, 0.1, 1, 10, 100 and 1000 ng/mL). After 45 minutes, cells that migrated were counted using a luminescent substrate and displayed on the bar graph above. Significant increases in migration over basal levels were seen in response to Rat MCP-1 starting at 10 ng/mL. This value is comparable to expected ranges of a chemotactic response of primary human monocytes.