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Proteins and Peptides

rRat GM-CSF Protein

Product Sizes
20 ug
£319.00
012-001-V12-0020-20UG
About this Product
SKU:
012-001-V12-0020
Additional Names:
Colony-stimulating factor (CSF), Pluripoietin-A Alpha, MGI1GM|csf2
Application:
Cell-based/Functional Assay, SDS-PAGE
Buffer:
See application note.
CE/IVD:
RUO
Extra Details:
Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) is hematopoietic factor produced by endothelial cells, monocytes, fibroblasts and T cells in response to a number of inflammatory mediators. GM-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages. Human and mouse GM-CSF show no cross-reactivity. Recombinant rat GM-CSF is a non-glycosylated protein, containing 128 amino acids, with a molecular weight of 14.6 kDa.
Immunogen:
csf2
Physical State:
Lyophilized
Purity:
>97%
Shipping Conditions:
Ambient
Specificity:
Granulocyte Macrophage-Colony Stimulating Factor purity was determined to be greater than 97% as determined by UV spectroscopy at 280 nm, Rp-HpLC calibrated against a known standard, and Quantitation against a known standard via reducing and non-reducing SDS-pAGE gels.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
SDS-PAGE of Rat Granulocyte Macrophage-Colony Stimulating Factor Recombinant Protein. Lane 1: 1 ug Rat GM-CSF in non-reducing conditions (-). Lane 2: Molecular weight marker. Lane 3: 1 ug Rat GM-CSF in reducing conditions (+). Lane 4: Molecular weight marker. Rat GM-CSF has a predicted MW of 14.5 kDa.
Bioactivity of Rat Granulocyte Macrophage-Colony Stimulating Factor Recombinant Protein. Serial dilutions of Rat GM-CSF, starting at 10 ng/mL, were added to FDCP-1 cells. After 91 hours, cell proliferation was measured and the linear portion of the curve was us used to calculate the ED50. The ED50 of Rat GM-CSF is 0.9-1.3 pg/mL. This value is comparable to the typical expected range of less than 0.01 ng/mL.