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Proteins and Peptides

rRat Leptin Protein

Product Sizes
1000 ug
£319.00
012-001-320-1000-1000UG
About this Product
SKU:
012-001-320-1000
Additional Names:
Lep|Obesity protein
Application:
Cell-based/Functional Assay, SDS-PAGE
Buffer:
0.1% Trifluoroacetic acid
CE/IVD:
RUO
Extra Details:
Leptin is a protein that is thought to have a critical role in the physiologic regulation of body weight via its capacity to inhibit food intake and stimulate energy expenditure. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Severe hereditary obesity in rodents and humans can be caused by defects in leptin production. These functions include the regulation of hematopoiesis, angiogenesis, wound healing, inflammation, and immune responses. Recombinant rat Leptin is a non-glycosylated protein, containing 147 amino acids, with a molecular weight of 16.2 kDa.
Formulation:
0.1% Trifluoroacetic acid
Immunogen:
Lep
Physical State:
Lyophilized
Purity:
>95%
Shipping Conditions:
Ambient
Specificity:
Leptin purity was determined to be greater than 95% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
SDS-PAGE of Rat Leptin Recombinant Protein. Lane 1: 1 ug Rat Leptin in non-reducing conditions (-). Lane 2: 1 ug Rat Leptin in reducing conditions (+). Lane 3: Molecular weight marker. Rat Leptin has a predicted MW of 16.2 kDa.
Bioactivity of Rat Leptin Recombinant Protein. Serial dilutions of Rat Leptin were added to Baf/3 cells. After 44 hours cell proliferation was measured and the linear portion of the curve was used to determine the ED50. The ED50 of Rat Leptin is 4-6.7 pg/mL. This value is comparable to the expected range of less than 5-10 pg/mL.