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Antibodies

Rabbit IgG Biotin conjugated

Product Sizes
1 mg
£187.00
011-0602-1MG
About this Product
SKU:
011-0602
Additional Names:
Rabbit IgG Biotin conjugation, Biotin conjugated Rabbit IgG whole molecule
Application:
ELISA, Flow Cytometry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Conjugate:
Biotin
Concentration:
1 mg/ml
Extra Details:
Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Rabbit IgG whole molecule Biotin Conjugated is ideal for investigators in Immunology, Cancer, and Microbiology research.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype:
IgG
Physical State:
Lyophilized
Shipping Conditions:
Ambient
Specificity:
Rabbit IgG whole molecule Biotin conjugated was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by extensive dialysis against the buffer stated above. Rabbit IgG whole molecule Biotin conjugated assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit IgG and anti-Rabbit Serum.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles., 2-8[o]C diluted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Isotype Controls
Identification and characterization of lung fibrocytes in IPF lungs. b Representative gating strategy for lung fibrocytes (CD45+CD15-CD3-CD19-CD56-CXCR4+Col-1+-cells) in explanted IPF lungs (n = 3 bottom) and control lungs (HC, top) (n = 4). Single cell suspensions were thawed and alive viable cells were further analyzed. Lung fibrocytes are present in the CD45+ cell population. Contaminating and unwanted CD15+ neutrophils, CD3+ T cells, CD19+ B cells and CD56+ NK-cells were sequentially excluded. Isotype control for Col-1 was used to set the gate for Col-1+ cells. To control for non-specific labeling Rabbit IgG-Biotin conjugated (p/n 011-0602) was used. Fig. 2. PMID: 29747640.
SDS-PAGE of Rabbit IgG Whole Molecule Rhodamine Conjugated (p/n 011-0002). Lane M: 3 uL Opal Prestained Marker (p/n MB-210-0500). Lane 1: Reduced Rabbit IgG Whole Molecule Rhodamine Conjugated (p/n 011-0002). Lane 2: Reduced Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Reduced Rabbit IgG F(c) Fragment (p/n 011-0103). Lane 4: Reduced Rabbit IgM Whole Molecule (p/n 011-0107). Load: 1 ug for F(ab) and F(c); 1.2 ug for IgG and IgM. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.