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Antibodies

Rabbit IgG F(ab')2

Product Sizes
2 mg
£237.00
011-0104-2MG
About this Product
SKU:
011-0104
Additional Names:
Rabbit Immunoglobulin IgG F(ab')2 fragment, IgG Fab2
Application:
ELISA, SDS-PAGE, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Concentration:
2 mg/ml
Extra Details:
Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype:
IgG
Physical State:
Lyophilized
Shipping Conditions:
Ambient
Specificity:
Rabbit IgG F(ab')2 was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG, anti-Rabbit IgG F(ab')2 and anti-Rabbit Serum. No reaction was observ
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles., -20[o]C/-70[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Isotype Controls
SDS PAGE gel of commercially-purified rabbit Immunoglobulin G (rIgG) and of rIgG papain digestion products Fab and Fc. Lanes C-F contain products which have been reduced with b-mercaptoethanol. Lanes H-L contain non-reduced products. Each lane contains approximately 35 mg of the following products: Lanes C and H contain papain digested products; Lanes D, I and L contain Fc fragment obtained from protein A purification; Lanes E and J contain whole rIgG; Lanes F and K contain Fab fragment obtained from protein A purification; Lanes A, B and G contain gel markers. Whole rIgG (p/n 011-0102), rFc (p/n 011-0103), rFab (p/n 011-0105), rF(ab')2 (p/n 011-0104). Fig. 3. PMID: 12643976.
SDS-PAGE of Rabbit IgG Whole Molecule Rhodamine Conjugated (p/n 011-0002). Lane M: 3 uL Opal Prestained Marker (p/n MB-210-0500). Lane 1: Reduced Rabbit IgG Whole Molecule Rhodamine Conjugated (p/n 011-0002). Lane 2: Reduced Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Reduced Rabbit IgG F(c) Fragment (p/n 011-0103). Lane 4: Reduced Rabbit IgM Whole Molecule (p/n 011-0107). Load: 1 ug for F(ab) and F(c); 1.2 ug for IgG and IgM. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.