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Antibodies

Rabbit IgG

Product Sizes
50 mg
£ POA
011-0102-50MG
About this Product
SKU:
011-0102
Additional Names:
Rabbit immunoglobulin G
Application:
ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Lateral Flow Assay, SDS-PAGE, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Concentration:
10 mg/ml
Extra Details:
Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype:
IgG
Physical State:
Lyophilized
Shipping Conditions:
Ambient
Specificity:
Rabbit IgG whole molecular was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Rabbit IgG whole molecular was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG and anti-Rabbit Serum.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles., 2-8[o]C diluted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Isotype Controls
SDS-PAGE of Rabbit IgG Whole Molecule. Lane 1: Non-reduced Rabbit IgG Whole Molecule. Lane 2: 5uL OPAL Pre-stained Marker (MB-210-0500). Lane 3: Reduced Rabbit IgG Whole Molecule. Load: 1ug per lane. Predicted/Observed size: Non-reduced at 150-170 kDa , Reduced at 55, 25 kDa.
Distribution of 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) within the pronucleus. Single images were obtained at different positions of the same pronucleus from z-stacks to illustrate the distribution of 5mC and 5hmC. The pattern of distribution of 5mC and 5hmC differs largely, with 5hmC being distributed homogeneously, while 5mC was concentrated in specific DNA regions. 5mC and 5hmC were evaluated in different zygotes. Zygotes were incubated with the non-immune control antibody rabbit IgG (p/n 011-0102). Fig. 5. PMID: 28331549.
Lateral flow test results for 58 serum samples, including 51 normal and 7 positive samples. [Symbol legend: (*) P < 0.05, (****) P < 0.0001 (one-way analysis of variance and Fisher's least significant difference test).] Mouse anti-human IgG antibody and rabbit IgG [p/n 011-0102] were combined, the mixture was dispensed onto the conjugate pad and dried. Figure 4. PMID: 32323974.
Representative sequences of the 15 identified groups from the NGS pool were screened for their individual binding abilities to Protein A. Comparative SPR-based interaction analyses were performed with the Biacore X100 instrument. Biotinylated aptamers were immobilized via the 5'-end (A) or 3'-end (B) on the streptavidin-modified sensor surface and 1000 nM Protein A was injected for binding. The sensor responses from the end of the binding phases (after 300 s) are shown. In addition, cross-specificities to other proteins were analyzed. Asterisks indicate if certain interactions have not been investigated. Rabbit IgG (p/n 011-0102). Figure 5. PMID: 29495282.
SDS PAGE gel of commercially-purified rabbit Immunoglobulin G (rIgG) and of rIgG papain digestion products Fab and Fc. Lanes A, B and G contain gel markers. Lanes C-F contain products which have been reduced with b-mercaptoethanol. Lanes H-L contain non-reduced products. Each lane contains approximately 35 mg of the following products: Lanes C and H contain papain digested products; Lanes D, I and L contain Fc fragment obtained from protein A purification; Lanes E and J contain whole rIgG; Lanes F and K contain Fab fragment obtained from protein A purification; Whole rIgG (p/n 011-0102), rFc (p/n 011-0103), rFab (p/n 011-0105), rF(ab')2 (p/n 011-0104). Fig. 3. PMID: 12643976.
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