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Antibodies

Mouse IgG Fab fragment

Product Sizes
2 mg
£246.00
010-0105-2MG
About this Product
SKU:
010-0105
Additional Names:
Mouse Immunoglobulin Fab, F(ab), Fragment antigen-binding
Application:
Bioactivity, Detection/Quantitation, ELISA, Immunofluorescence, Immunohistochemistry, SDS-PAGE, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Concentration:
2 mg/ml
Extra Details:
Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype:
IgG
Physical State:
Liquid
Shipping Conditions:
Blue Ice
Specificity:
Mouse IgG Fab fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Mouse IgG Fab fragment assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG, anti-Mouse IgG F(ab')2 and anti-Mouse Ser
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles., 2-8[o]C diluted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Isotype Controls
Ability of ProteoChip to bind the antibody F(c) region. (b) Scanning images of protein microarray: competition between FITC-labeled F(c) [p/n 010-0203] and unlabeled Fab fragments [p/n 010-0105] (upper picture), and FITC-labeled F(c) [p/n 010-0203] and unlabeled F(c) fragments [010-0103] (lower picture). (c) Scanning images were analyzed using QuantumArray software, and fluorescence intensities of each spot were plotted versus competitor concentration. Competition between FITC-labeled F(c) and unlabeled Fab fragment, and FITC-labeled F(c) and unlabeled F(c) fragments, are shown by the solid line and broken line, respectively. Figure 2. PMID: 15538771.
Characterization of the anti-IgG nanobody toolbox. (a) Overview of all identified anti-IgG nanobodies. The nanobodies obtained were characterized for IgG subclass and light chain specificity, epitope location on Fab or Fc fragment, and species cross reactivity. The protein sequences of all anti-IgG nanobodies can be found in Table S1. Nb, nanobody; CDR III, complementarity-determining region III; Gp, guinea pig; Hs, human; K Kappa, K Kappa light chain; L Lambda, lambda light chain; Fab, fragment antigen-binding, Fc, fragment crystallizable. (a. not shown) (b) IgG subclass reactivity profiling of selected anti-mouse IgG nanobodies representing all identified specificity groups. The indicated IgG species were spotted on nitrocellulose strips, and the strips were blocked with 4% (wt/vol) milk in 1x PBS. Then 300 nM of the indicated tagged nanobodies were added in milk. After washing with 1x PBS, bound nanobodies were detected using a fluorescence scanner. Note that the signal strength on polyclonal IgG depends on the relative abundance of the specific subclass (e.g., IgG2b and IgG3 are low abundance) or light chain (K Kappa/L Lambda ratio = 99:1). TP885 and TP926 showed no detectable binding to polyclonal Fab or Fc fragment and might bind to the hinge region. (p/n 010-001-331 Mouse IgG1 L Lambda lambda, 010-0105 Mouse Fab fragment). Fig 1. PMID: 29263082.
SDS-PAGE of Mouse IgG Whole Molecule Rhodamine Conjugated (p/n 010-0002). MW: 5 uL Opal Prestained Marker (p/n MB-210-0500). Lane 1: Reduced Mouse IgG Whole Molecule Rhodamine Conjugated (p/n 010-0002). Lane 2: Reduced Mouse F(c) Fragment (p/n 010-0103). Lane 3: Reduced Mouse F(ab) Fragment (p/n 010-0105). Lane 4: Mouse IgM Kappa Myeloma Protein (p/n 010-001-033). Load: 1 ug per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.