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Proteins and Peptides

rMouse Thrombopoietin Protein

Product Sizes
10 ug
£319.00
010-001-W32-0010-10UG
About this Product
SKU:
010-001-W32-0010
Additional Names:
C-mpl ligand (ML), Megakaryocyte Colony Stimulating Factor, Megakaryocyte growth and development factor (MGDF), Myeloproliferative leukemia virus oncogene ligan, c-MPL ligand|Thpo
Application:
Cell-based/Functional Assay, SDS-PAGE
Buffer:
0.01 M Sodium Phosphate
CE/IVD:
RUO
Extra Details:
Thrombopoietin (TPO) is a growth factor that is produced by the liver and kidney. TPO acts through the TPO receptor to promote megakaryocyte maturation and differentiation, which leads to the production of platelets. Recombinant mouse TPO is a non-glycosylated protein, containing 174 amino acids (which comprise the receptor binding domain), with a molecular weight of 18.7 kDa.
Formulation:
0.01 M Sodium Phosphate, pH 7.5
Immunogen:
Thpo
Physical State:
Lyophilized
Purity:
>97%
Shipping Conditions:
Ambient
Specificity:
Thrombopoietin purity was determined to be greater than 97% as determined by reducing and non-reducing SDS-pAGE.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
SDS-PAGE of Mouse Thrombopoietin Recombinant Protein. Lane 1: Molecular weight marker. Lane 2: 1 ug Mouse TPO in non-reducing conditions (-). Lane 3: Molecular weight marker. Lane 4: 1 ug Mouse TPO in reducing conditions (+). Mouse TPO has a predicted MW of 18.7 kDa.
Bioactivity of Mouse Thrombopoietin Recombinant Protein. Serial dilutions of Mouse TPO, starting at 200 ng/mL, were added to MO7e cells growing in the presence of 1 ng/mL IL-3 and 2.5 ng/mL SCF. Proliferation was measure after 5 days and the linear portion of the curve was us used to calculate the ED50. The ED50 of Mouse TPO is less than 0.8 ng/mL. This value is comparable to the typical expected range of 1 ng/mL.