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Proteins and Peptides

rMouse SDF-1 alpha/CXCL12 Protein

Product Sizes
10 ug
£319.00
010-001-W22-0010-10UG
About this Product
SKU:
010-001-W22-0010
Additional Names:
12-O-tetradecanoylphorbol 13-acetate repressed protein 1 (TPAR1), C-X-C motif chemokine 12, Pre-B cell growth-stimulating factor (PBSF), Thymic lymphoma cell-stimulating factor (TLSF)|Cxcl12
Application:
Cell-based/Functional Assay, SDS-PAGE
Buffer:
0.1% Trifluoroacetic acid
CE/IVD:
RUO
Concentration:
0.1 mg/ml
Extra Details:
Stromal Cell Derived Factor-1 alpha (SDF-1A Alpha), also called CXCL12, is one of two splice variants made by a wide variety of cells when stimulated by inflammatory cytokines such as, TNF, IL-1 or LPS. SDF-1A Alpha signals through the G protein-couple receptor, CXCR4, to recruit activated leukocytes. Human and mouse SDF-1A Alpha share 99% sequence identity. Recombinant mouse SDF-1A Alpha is a non-glycosylated protein, containing 68 amino acids, with a molecular weight of 7.9 kDa.
Formulation:
0.1% Trifluoroacetic acid
Immunogen:
Cxcl12
Physical State:
Lyophilized
Purity:
>98%
Shipping Conditions:
Ambient
Specificity:
Stromal Cell-Derived Factor-1 alpha (CXCL12) purity was determined to be greater than 98% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
SDS-PAGE of Mouse Stromal Cell-Derived Factor-1 alpha (CXCL12) Recombinant Protein. Lane 1: 1 ug Mouse SDF-1 alpha in non-reducing conditions (-). Lane 2: Molecular weight marker. Lane 3: 1 ug Mouse SDF-1 alpha in reducing conditions (+). Lane 4: Molecular weight marker. Mouse SDF-1 alpha has a predicted MW of 7.9 kDa.
Bioactivity of Mouse Stromal Cell-Derived Factor-1 alpha (CXCL12) Recombinant Protein. Samples of primary naive human CD3+ T cells were allowed to migrate to Mouse SDF-1A Alpha/CXCL12 (0, 5, 100, and 500 ng/mL). After 4 hours, cells that migrated were counted using a fluorescent substrate and displayed on the bar graph above. Increased levels of migration over basal were seen in response to Mouse SDF-1A Alpha/CXCL12 starting at 5 ng/mL. This result is comparable to the expected range of 10 ng/mL.