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Proteins and Peptides

rMouse PDGF-AA Protein

Product Sizes
10 ug
£319.00
010-001-W08-0010-10UG
About this Product
SKU:
010-001-W08-0010
Additional Names:
PDGF-1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha polypeptide|Pdgfa
Application:
Cell-based/Functional Assay, SDS-PAGE
Buffer:
0.1% Trifluoroacetic acid
CE/IVD:
RUO
Extra Details:
Platelet-Derived Growth Factor (PDGF) is a mitogenic peptide growth hormone carried in the alpha-granules of platelets and is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication. The synthesis of PDGF can be induced by IL-1, IL-6, TNF-A Alpha, TGF-B Beta and EGF. Recombinant mouse PDGF-AA is a non-glycosylated, disulfide-linked homodimer, containing two 126 amino acid chains, with a total molecular weight of 28.9 kDa.
Formulation:
0.1% Trifluoroacetic acid
Immunogen:
Pdgfa
Physical State:
Lyophilized
Purity:
>97%
Shipping Conditions:
Ambient
Specificity:
platelet Derived Growth Factor AA purity was determined to be greater than 97% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
SDS-PAGE of Mouse Platelet Derived Growth Factor-AA Recombinant Protein. Lane 1: 1 ug Mouse PDGF-AA in non-reducing conditions (-). Lane 2: 1 ug Mouse PDGF-AA in reducing conditions (+). Lane 3: Molecular weight marker. Mouse PDGF AA is a homodimer having a predicted MW totaling 28.9 kDa.
Bioactivity of Mouse Platelet Derived Growth Factor AA Recombinant Protein. Serial dilutions of Mouse PDGF-AA, starting at 100 ng/mL, were added to NIH 3T3 cells. After 40 hours, cell viability was measured and the linear portion of the curve was us used to calculate the ED50. The ED50 of Mouse PDGF-AA is 3.4-5 ng/mL. This value is comparable to the typical expected range of 3-5 ng/mL.